nm23-H1 reduces in vitro cell migration and the liver metastatic potential of colon cancer cells by regulating myosin light chain phosphorylation

Eiji Suzuki; Tetsuya Ota; Kazunori Tsukuda; Atsushi Okita; Kinya Matsuoka; Masakazu Murakami; Hiroyoshi Doihara; Nobuyoshi Shimizu

doi:10.1002/ijc.11546

nm23-H1 reduces in vitro cell migration and the liver metastatic potential of colon cancer cells by regulating myosin light chain phosphorylation

Eiji Suzuki, Tetsuya Ota, Kazunori Tsukuda, Atsushi Okita, Kinya Matsuoka, Masakazu Murakami, Hiroyoshi Doihara, Nobuyoshi Shimizu

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

Abstract

The nm23-H1 gene is known as a potential metastasis suppressor gene in various types of carcinomas. However, the role of nm23-H1 in colorectal carcinoma still remains controversial and the cellular mechanisms by which its protein may modulate the metastatic phenotype are not yet known. We transfected nm23-H1 cDNA into the human colon cancer cell line, HT-29, to test the effects and cellular biological mechanism of nm23 protein in colon cancer. We found that nm23-H1 strongly inhibited the liver metastasis of HT-29 cells in nude mice and inhibited the epidermal growth factor (EGF)-induced cell migration in vitro. Furthermore, we clarified the regulation of the myosin light chain (MLC) phosphorylation by nm23-H1, which has been demonstrated as having potential role in cell migration.

Original language	English
Pages (from-to)	207-211
Number of pages	5
Journal	International Journal of Cancer
Volume	108
Issue number	2
DOIs	https://doi.org/10.1002/ijc.11546
Publication status	Published - Jan 10 2004
Externally published	Yes

Keywords

Colon cancer cell
MAPK
Migration
Myosin light chain
nm23-H1

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/ijc.11546

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title = "nm23-H1 reduces in vitro cell migration and the liver metastatic potential of colon cancer cells by regulating myosin light chain phosphorylation",

abstract = "The nm23-H1 gene is known as a potential metastasis suppressor gene in various types of carcinomas. However, the role of nm23-H1 in colorectal carcinoma still remains controversial and the cellular mechanisms by which its protein may modulate the metastatic phenotype are not yet known. We transfected nm23-H1 cDNA into the human colon cancer cell line, HT-29, to test the effects and cellular biological mechanism of nm23 protein in colon cancer. We found that nm23-H1 strongly inhibited the liver metastasis of HT-29 cells in nude mice and inhibited the epidermal growth factor (EGF)-induced cell migration in vitro. Furthermore, we clarified the regulation of the myosin light chain (MLC) phosphorylation by nm23-H1, which has been demonstrated as having potential role in cell migration.",

keywords = "Colon cancer cell, MAPK, Migration, Myosin light chain, nm23-H1",

author = "Eiji Suzuki and Tetsuya Ota and Kazunori Tsukuda and Atsushi Okita and Kinya Matsuoka and Masakazu Murakami and Hiroyoshi Doihara and Nobuyoshi Shimizu",

year = "2004",

month = jan,

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doi = "10.1002/ijc.11546",

language = "English",

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T1 - nm23-H1 reduces in vitro cell migration and the liver metastatic potential of colon cancer cells by regulating myosin light chain phosphorylation

AU - Suzuki, Eiji

AU - Ota, Tetsuya

AU - Tsukuda, Kazunori

AU - Okita, Atsushi

AU - Matsuoka, Kinya

AU - Murakami, Masakazu

AU - Doihara, Hiroyoshi

AU - Shimizu, Nobuyoshi

PY - 2004/1/10

Y1 - 2004/1/10

N2 - The nm23-H1 gene is known as a potential metastasis suppressor gene in various types of carcinomas. However, the role of nm23-H1 in colorectal carcinoma still remains controversial and the cellular mechanisms by which its protein may modulate the metastatic phenotype are not yet known. We transfected nm23-H1 cDNA into the human colon cancer cell line, HT-29, to test the effects and cellular biological mechanism of nm23 protein in colon cancer. We found that nm23-H1 strongly inhibited the liver metastasis of HT-29 cells in nude mice and inhibited the epidermal growth factor (EGF)-induced cell migration in vitro. Furthermore, we clarified the regulation of the myosin light chain (MLC) phosphorylation by nm23-H1, which has been demonstrated as having potential role in cell migration.

AB - The nm23-H1 gene is known as a potential metastasis suppressor gene in various types of carcinomas. However, the role of nm23-H1 in colorectal carcinoma still remains controversial and the cellular mechanisms by which its protein may modulate the metastatic phenotype are not yet known. We transfected nm23-H1 cDNA into the human colon cancer cell line, HT-29, to test the effects and cellular biological mechanism of nm23 protein in colon cancer. We found that nm23-H1 strongly inhibited the liver metastasis of HT-29 cells in nude mice and inhibited the epidermal growth factor (EGF)-induced cell migration in vitro. Furthermore, we clarified the regulation of the myosin light chain (MLC) phosphorylation by nm23-H1, which has been demonstrated as having potential role in cell migration.

KW - Colon cancer cell

KW - MAPK

KW - Migration

KW - Myosin light chain

KW - nm23-H1

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DO - 10.1002/ijc.11546

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VL - 108

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JO - International Journal of Cancer

JF - International Journal of Cancer

IS - 2

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nm23-H1 reduces in vitro cell migration and the liver metastatic potential of colon cancer cells by regulating myosin light chain phosphorylation

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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