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nm23-H1 reduces in vitro cell migration and the liver metastatic potential of colon cancer cells by regulating myosin light chain phosphorylation

  • Eiji Suzuki
  • , Tetsuya Ota
  • , Kazunori Tsukuda
  • , Atsushi Okita
  • , Kinya Matsuoka
  • , Masakazu Murakami
  • , Hiroyoshi Doihara
  • , Nobuyoshi Shimizu

Research output: Contribution to journalArticlepeer-review

Abstract

The nm23-H1 gene is known as a potential metastasis suppressor gene in various types of carcinomas. However, the role of nm23-H1 in colorectal carcinoma still remains controversial and the cellular mechanisms by which its protein may modulate the metastatic phenotype are not yet known. We transfected nm23-H1 cDNA into the human colon cancer cell line, HT-29, to test the effects and cellular biological mechanism of nm23 protein in colon cancer. We found that nm23-H1 strongly inhibited the liver metastasis of HT-29 cells in nude mice and inhibited the epidermal growth factor (EGF)-induced cell migration in vitro. Furthermore, we clarified the regulation of the myosin light chain (MLC) phosphorylation by nm23-H1, which has been demonstrated as having potential role in cell migration.

Original languageEnglish
Pages (from-to)207-211
Number of pages5
JournalInternational Journal of Cancer
Volume108
Issue number2
DOIs
Publication statusPublished - Jan 10 2004
Externally publishedYes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

Keywords

  • Colon cancer cell
  • MAPK
  • Migration
  • Myosin light chain
  • nm23-H1

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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