TY - JOUR
T1 - NMR signal assignment of the polyuridine tract of the single-stranded RNA complexed with Sxl RBD1-RBD2 by using residue selective [5-(2)H]uridine substitutions.
AU - Kim, I.
AU - Muto, Y.
AU - Hosono, K.
AU - Kitamura, A.
AU - Watanabe, S.
AU - Ohtsuki, T.
AU - Kawai, G.
AU - Takaku, H.
AU - Watanabe, K.
AU - Sakamoto, H.
AU - Shimura, Y.
AU - Yokoyama, S.
PY - 1999
Y1 - 1999
N2 - Using [5-(2)H]uridine phosphoramidite, we synthesized a series of 2H-labeled Drosophila Sex-lethal (Sxl) target RNAs, in which all the uridine residues except one were specifically replaced by [5-(2)H]uridine. By observing the H5-H6 cross peaks of RNA in the TOCSY spectra, we unambiguously assigned all the base proton resonances of the target RNA in a Sxl x RNA complex. Furthermore, it was shown that Sxl differently recognizes A and G in a position prior to the polyuridine tract.
AB - Using [5-(2)H]uridine phosphoramidite, we synthesized a series of 2H-labeled Drosophila Sex-lethal (Sxl) target RNAs, in which all the uridine residues except one were specifically replaced by [5-(2)H]uridine. By observing the H5-H6 cross peaks of RNA in the TOCSY spectra, we unambiguously assigned all the base proton resonances of the target RNA in a Sxl x RNA complex. Furthermore, it was shown that Sxl differently recognizes A and G in a position prior to the polyuridine tract.
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U2 - 10.1093/nass/42.1.203
DO - 10.1093/nass/42.1.203
M3 - Article
C2 - 10780450
AN - SCOPUS:17444435253
SN - 0261-3166
SP - 203
EP - 204
JO - Nucleic acids symposium series
JF - Nucleic acids symposium series
IS - 42
ER -