TY - JOUR
T1 - Non-thiol-activated property of a cholesterol-binding hemolysin produced by Vibrio vulnificus
AU - Miyoshi, Shin ichi
AU - Yamanaka, Hiroyasu
AU - Miyoshi, Noriko
AU - Shinoda, Sumio
PY - 1985
Y1 - 1985
N2 - Vibrio vulnificus hemolysin (VVH) and streptolysin O (SLO) are both cholesterol-binding hemolysins. Both hemolysins were inactivated with H2O2, but the lost activity of SLO was restored by addition of thiol compounds, whereas that of VVH was not. Moreover, the activity of VVH was lowered by thiol compounds but not by thiolblocking agents, whereas the latter produced a decrease in SLO activity. These results suggest that VVH is not a thiol-activated hemolysin, in spite of its cholesterol-binding property.
AB - Vibrio vulnificus hemolysin (VVH) and streptolysin O (SLO) are both cholesterol-binding hemolysins. Both hemolysins were inactivated with H2O2, but the lost activity of SLO was restored by addition of thiol compounds, whereas that of VVH was not. Moreover, the activity of VVH was lowered by thiol compounds but not by thiolblocking agents, whereas the latter produced a decrease in SLO activity. These results suggest that VVH is not a thiol-activated hemolysin, in spite of its cholesterol-binding property.
KW - Marine vibrio
KW - cytolysin
KW - oxygen-labile hemolysin
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U2 - 10.1111/j.1574-6968.1985.tb01014.x
DO - 10.1111/j.1574-6968.1985.tb01014.x
M3 - Article
AN - SCOPUS:0022365216
SN - 0378-1097
VL - 30
SP - 213
EP - 216
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1-2
ER -