Non-viral gene delivery regulated by stiffness of cell adhesion substrates

Hyun Joon Kong; Jodi Liu; Kathryn Riddle; Takuya Matsumoto; Kent Leach; David J. Mooney

doi:10.1038/nmat1392

Non-viral gene delivery regulated by stiffness of cell adhesion substrates

Hyun Joon Kong, Jodi Liu, Kathryn Riddle, Takuya Matsumoto, Kent Leach, David J. Mooney

Research output: Contribution to journal › Article › peer-review

214 Citations (Scopus)

Abstract

The rigidity of the cell adhesion substrate on the level of gene transfer and expression, was investigated. Gene-transfer efficiency was investigated using a fluorescent resonance energy transfer (FRET) technique, as FRET techniques have been extensively used to evaluate specific intermolecular interactions at the nanometre scale. The mechanical properties of the substrate to which cell adheres have been found to mediate many aspects of cell function including proliferation, migration and differentiations. The study indicated the importance of material mechanics in regulating gene transfer and expression, and also enabled to understand the gene transfer processes in different tissues.

Original language	English
Pages (from-to)	460-464
Number of pages	5
Journal	Nature Materials
Volume	4
Issue number	6
DOIs	https://doi.org/10.1038/nmat1392
Publication status	Published - Jun 2005
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)
Materials Science(all)
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

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10.1038/nmat1392

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title = "Non-viral gene delivery regulated by stiffness of cell adhesion substrates",

abstract = "The rigidity of the cell adhesion substrate on the level of gene transfer and expression, was investigated. Gene-transfer efficiency was investigated using a fluorescent resonance energy transfer (FRET) technique, as FRET techniques have been extensively used to evaluate specific intermolecular interactions at the nanometre scale. The mechanical properties of the substrate to which cell adheres have been found to mediate many aspects of cell function including proliferation, migration and differentiations. The study indicated the importance of material mechanics in regulating gene transfer and expression, and also enabled to understand the gene transfer processes in different tissues.",

author = "Kong, {Hyun Joon} and Jodi Liu and Kathryn Riddle and Takuya Matsumoto and Kent Leach and Mooney, {David J.}",

note = "Funding Information: We thank Michael Solomon at the University of Michigan for use of the laser scanning confocal microscope. We also thank Yoshiaki Hirano at Osaka University for helpful discussions. Financial support was provided by the National Institute of Health (R30 DE 13033). Correspondence and requests for materials should be addressed to D.J.M. Supplementary Information accompanies the paper on www.nature.com/naturematerials.",

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AU - Kong, Hyun Joon

AU - Liu, Jodi

AU - Riddle, Kathryn

AU - Matsumoto, Takuya

AU - Leach, Kent

AU - Mooney, David J.

N1 - Funding Information: We thank Michael Solomon at the University of Michigan for use of the laser scanning confocal microscope. We also thank Yoshiaki Hirano at Osaka University for helpful discussions. Financial support was provided by the National Institute of Health (R30 DE 13033). Correspondence and requests for materials should be addressed to D.J.M. Supplementary Information accompanies the paper on www.nature.com/naturematerials.

PY - 2005/6

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AB - The rigidity of the cell adhesion substrate on the level of gene transfer and expression, was investigated. Gene-transfer efficiency was investigated using a fluorescent resonance energy transfer (FRET) technique, as FRET techniques have been extensively used to evaluate specific intermolecular interactions at the nanometre scale. The mechanical properties of the substrate to which cell adheres have been found to mediate many aspects of cell function including proliferation, migration and differentiations. The study indicated the importance of material mechanics in regulating gene transfer and expression, and also enabled to understand the gene transfer processes in different tissues.

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Non-viral gene delivery regulated by stiffness of cell adhesion substrates

Abstract

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