Nondestructive assessment of current one-step self-etch dental adhesives using optical coherence tomography

Baba Bista, Alireza Sadr, Amir Nazari, Yasushi Shimada, Yasunori Sumi, Junji Tagami

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)


This study aimed to nondestructively evaluate sealing performance of eight one-step self-etch adhesives (1-SEAs) using optical coherence tomography (OCT). The two-step self-etch adhesive (2-SEA) served as the control. Round tapered class-I cavities (D = 4 mm, H = 2 mm) were prepared in bovine incisors, treated with each adhesive (n = 5), and restored with a flowable resin composite. Cross-sections were obtained from each restoration using swept-source OCT with 1310-nm laser. The average percentage of the sealed interface (SI%) for each adhesive was calculated using image analysis software, considering increased signal intensity at the interface as gap. Samples were then sectioned and observed under confocal laser scanning microscope (CLSM). Significantly different SI% values were found among different adhesives (analysis of variance, Bonferroni, p < 0.05). There was also a significant correlation in SI% between OCT and CLSM (p < 0.0001, r = 0.96). Additionally, microscopic analysisrevealed that the gaps in 1-SEAs occurred not only at dentine-adhesive interfaces but also frequently at adhesive-composite interfaces. Some recent 1-SEAs could achieve reliable short-term sealing comparable to 2-SEA. OCT is a unique tool to nondestructively evaluate the sealing performance of the restoratives through the cavity, provided that cavity walls have a certain minimum inclination with respect to the beam.

Original languageEnglish
Article number076020
JournalJournal of Biomedical Optics
Issue number7
Publication statusPublished - Jul 2013
Externally publishedYes


  • adaptation
  • attenuation
  • confocal laser scanning microscope.
  • image analysis
  • optical coherence tomography
  • refractive index
  • self-etch adhesives

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering


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