@article{ac4f9b8d9e534107903d5428e90b0252,
title = "Novel assay system for acidic Peptide:N-glycanase (aPNGase) activity in crude plant extract",
abstract = "Acidic peptide:N-glycanase (aPNGase) plays a pivotal role in plant glycoprotein turnover. For the construction of aPNGase-knockout or -overexpressing plants, a new method to detect the activity in crude plant extracts is required because endogenous peptidases present in the extract hamper enzyme assays using fluorescence-labeled N-glycopeptides as a substrate. In this study, we developed a new method for measuring aPNGase activity in crude extracts from plant materials.",
keywords = "Acidic Pngase, Affinity chromatography, Enzyme assay, Free N-glycans, Transgenic plant",
author = "Ryota Uemura and Mikako Ogura and Chihiro Matsumaru and Tsuyoshi Akiyama and Megumi Maeda and Yoshinobu Kimura",
note = "Funding Information: This work was supported in part by grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan [(Basic Research C No.15K07841 and Fostering Joint International Research No.15KK0282 to M.M., and No. 17K0819709 to YK)], and the Research Grants of Mizutani Foundation for Glycoscience (to Y.K.). Funding Information: We are grateful to the Department of Instrumental Analysis, Advanced Science Research Center, Okayama University, for ESI-MS analysis. This work was supported in part by grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan [(Basic Research C No.15K07841 and Fostering Joint International Research No.15KK0282 to M.M., and No. 17K0819709 to YK)], and the Research Grants of Mizutani Foundation for Glycoscience (to Y.K.). Publisher Copyright: {\textcopyright} 2018 Japan society for Bioscience, Biotechnology, and agrochemistry.",
year = "2018",
doi = "10.1080/09168451.2018.1459464",
language = "English",
volume = "82",
pages = "1172--1175",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "7",
}