@inbook{41a9572044c34489b087b01fc68a1ffa,
title = "Novel Cell Biological Assays for Measuring Bone Remodeling Activities of CCN Proteins",
abstract = "Although two-dimensional (2D) cultures from bone lineage cells are often used, it is well-known that this culture system is completely different from the in vivo bone matrix environment. In this paper, we describe a 3D culture method using both the mouse osteocytic cell line, MLO-Y4, and an osteocyte-enriched population of the cells isolated from mice. These cells are embedded in collagen gel with recombinant cellular communication network (CCN) factor proteins; then, osteoblasts or osteoclasts are inoculated and cultured on the collagen gel. Because this method mimics the in vitro bone matrix environment, it is useful for understanding the detailed mechanism of actions of CCN proteins in the bone matrix.",
keywords = "3D culture, Bone remodeling, Osteoblast differentiation, Osteoclastogenesis, Osteocytes, Recombinant CCN2 protein (rCCN2)",
author = "Takashi Nishida and Satoshi Kubota and Masaharu Takigawa",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2744-0_17",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "255--268",
booktitle = "Methods in Molecular Biology",
}