Novel in vitro screening system for monoclonal antibodies using hypermutating chicken B cell library

Kagefumi Todo; Kenji Miyake; Masaki Magari; Naoki Kanayama; Hitoshi Ohmori

doi:10.1263/jbb.102.478

Novel in vitro screening system for monoclonal antibodies using hypermutating chicken B cell library

Kagefumi Todo, Kenji Miyake, Masaki Magari, Naoki Kanayama, Hitoshi Ohmori

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Here, we report an in vitro screening system for monoclonal antibodies using a hypermutating chicken B cell line, DT40-SW. When switching on hypermutation, cultured DT40-SW cells constituted an antibody library, from which clones secreting antibodies to a test antigen were successfully isolated, and genetically stabilized by switching off the mutation machinery.

Original language	English
Pages (from-to)	478-481
Number of pages	4
Journal	Journal of Bioscience and Bioengineering
Volume	102
Issue number	5
DOIs	https://doi.org/10.1263/jbb.102.478
Publication status	Published - Nov 2006

Keywords

chicken B cell line
hypermutation
monoclonal antibody

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1263/jbb.102.478

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title = "Novel in vitro screening system for monoclonal antibodies using hypermutating chicken B cell library",

abstract = "Here, we report an in vitro screening system for monoclonal antibodies using a hypermutating chicken B cell line, DT40-SW. When switching on hypermutation, cultured DT40-SW cells constituted an antibody library, from which clones secreting antibodies to a test antigen were successfully isolated, and genetically stabilized by switching off the mutation machinery.",

keywords = "chicken B cell line, hypermutation, monoclonal antibody",

author = "Kagefumi Todo and Kenji Miyake and Masaki Magari and Naoki Kanayama and Hitoshi Ohmori",

note = "Funding Information: This work was partially supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by the Industrial Technology Research Grant Program in 2005 from New Energy and Industrial Technology Development Organization (NEDO) of Japan. K.T. was supported by Research Fellowships from the Japan Society for the Promotion of Science (JSPS) for Young Scientists.",

year = "2006",

month = nov,

doi = "10.1263/jbb.102.478",

language = "English",

volume = "102",

pages = "478--481",

journal = "Journal of Bioscience and Bioengineering",

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publisher = "The Society for Biotechnology, Japan",

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T1 - Novel in vitro screening system for monoclonal antibodies using hypermutating chicken B cell library

AU - Todo, Kagefumi

AU - Miyake, Kenji

AU - Magari, Masaki

AU - Kanayama, Naoki

AU - Ohmori, Hitoshi

N1 - Funding Information: This work was partially supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by the Industrial Technology Research Grant Program in 2005 from New Energy and Industrial Technology Development Organization (NEDO) of Japan. K.T. was supported by Research Fellowships from the Japan Society for the Promotion of Science (JSPS) for Young Scientists.

PY - 2006/11

Y1 - 2006/11

N2 - Here, we report an in vitro screening system for monoclonal antibodies using a hypermutating chicken B cell line, DT40-SW. When switching on hypermutation, cultured DT40-SW cells constituted an antibody library, from which clones secreting antibodies to a test antigen were successfully isolated, and genetically stabilized by switching off the mutation machinery.

AB - Here, we report an in vitro screening system for monoclonal antibodies using a hypermutating chicken B cell line, DT40-SW. When switching on hypermutation, cultured DT40-SW cells constituted an antibody library, from which clones secreting antibodies to a test antigen were successfully isolated, and genetically stabilized by switching off the mutation machinery.

KW - chicken B cell line

KW - hypermutation

KW - monoclonal antibody

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AN - SCOPUS:33845664766

SN - 1389-1723

VL - 102

SP - 478

EP - 481

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

IS - 5

ER -

Novel in vitro screening system for monoclonal antibodies using hypermutating chicken B cell library

Abstract

Keywords

ASJC Scopus subject areas

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