Novel method for evaluation of anaerobic germination in rice and its application to diverse genetic collections

Noriyuki Kuya, Jian Sun, Ken Iijima, Ramaiah Venuprasad, Toshio Yamamoto

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Direct seeding saves time and labour in the cultivation of rice. However, seedling establishment is often unstable, and yields are lower than in transplanting. Anaerobic germination (AG) is a key trait for improvement of direct seeding of rice. We established a simple and reliable method of evaluating AG in rice breeding. We germinated seeds in distilled water or deoxygenated water and measured coleoptile length several days later; compared the results of each method with survival rate in flooded soil; and used the anoxic water method for QTL analysis and for testing cultivars. Coleoptile elongation in anoxic water and survival rate in flooded soil were significantly correlated (r = 0.879, P < 0.01). A significant QTL, likely to be a major gene (AG1), was found in chromosome segment substitution lines and in a backcrossed F2 population derived from tolerant and sensitive lines. Diverse rice genetic resources were classified into tolerant or sensitive accession groups reflecting their ecotypes. Our study revealed that anoxic water evaluation method saves space and time in a stable environment compared with flooded soil evaluation. It is applicable to QTL analysis and isolation of genes underlying anaerobic germination.

Original languageEnglish
Pages (from-to)633-639
Number of pages7
JournalBreeding Science
Issue number4
Publication statusPublished - 2019


  • Anaerobic germination
  • Anoxic water
  • Direct seeding
  • Genetic resources
  • Phenotyping method
  • QTL
  • Rice

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science


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