@inbook{63b392308c204b05a7eacfd3d24b93ee,
title = "Numerical Simulation of Shaking Optimization in a Suspension Culture of iPS Cells",
abstract = "Research on induced Pluripotent Stem (iPS) cell has attracted attention due to their remarkable progress in regenerative medicine. Cost and quality are two main factors to be considered when using iPS cells in biological applications in a large scale. In this study, the suspension culture of iPS cells was numerically simulated in a cylindrical tank under the two-different shaking methods; one-direction rotation and periodic alternate rotation. The two shaking methods exhibited a significant difference in the average number of cells accumulated in the bottom. Even though the one direction rotation method suppressed the shear stress acting on iPS cells, after eight seconds it accumulated more cells in the bottom than the periodic alternate rotation method.",
keywords = "iPS cell, Numerical simulation, Suspension culture",
author = "Kelum Elvitigala and Yoshiki Kanemaru and Masaki Yano and Atsushi Sekimoto and Yasunori Okano and Masahiro Kino-Oka",
note = "Funding Information: Acknowledgements. This research work was financially supported by the project of “Development of Cell Production and Processing System for Commercialization of Regenerative Medicine” from Japan Agency for Medical Research and Development, AMED, and by Grant-in-Aid for Scientific Research (B) (JSPS KAKENHI Grant Number JP15H04173) from Ministry of Education, Culture, Sports, Science and Technology of Japan, and supported partly by Collaborate Research Program for Young Scientists of ACCMS and IIMC, Kyoto University. Publisher Copyright: {\textcopyright} 2019, Springer Nature Switzerland AG.",
year = "2019",
doi = "10.1007/978-3-319-99834-3_37",
language = "English",
series = "Lecture Notes in Networks and Systems",
publisher = "Springer",
pages = "283--289",
booktitle = "Lecture Notes in Networks and Systems",
}