TY - JOUR
T1 - Occurrence, infectivity and molecular characterization of hosta virus X in North-east China
AU - Song, Shuang
AU - Cui, Jie
AU - Lei, Gui Jie
AU - Chen, Yu Fei
AU - Yang, Ming Xiu
AU - Li, Zheng Nan
AU - Zhang, Jun Hua
N1 - Funding Information:
This work was supported by the Natural Science Foundation of Heilongjiang Province, China [LH2019C024]. We are grateful to Dr Qiang Wang of the University of Florida for his help in improving the English of this manuscript.
Publisher Copyright:
© 2020 The Canadian Phytopathological Society.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Hosta virus X (HVX) is the most economically significant virus infecting hosta (Hosta spp.) plants worldwide, and has been reported from North America, Europe, Asia and Oceania. In May 2019, various viral-like disease symptoms were observed on hosta plants in a park in Harbin City in North-east China, and infection by HVX was confirmed by small RNA sequencing, RT-PCR and electron microscopy. The infectivity of HVX on Hosta ensata, a native species in North-east China, was confirmed by mechanical inoculation. We determined the complete genome sequence of an isolate of the virus, which revealed a high level of sequence similarity with other HVX genome sequences published to date. Phylogenetic analysis of HVX with other potexviruses based on the complete sequences revealed that all four HVX entries clustered to a single clade, which was most closely related to cassava common mosaic virus (CsCMV), hydrangea ringspot virus (HdRSV), plantago asiatica mosaic virus (PlAMV) and tulip virus X (TVX). Two potential recombination events were detected among the four HVX isolates. This work not only reports the complete genome sequence of an HVX isolate in North-east China for the first time, but also suggests the need to prevent further spread of this virus in the region.
AB - Hosta virus X (HVX) is the most economically significant virus infecting hosta (Hosta spp.) plants worldwide, and has been reported from North America, Europe, Asia and Oceania. In May 2019, various viral-like disease symptoms were observed on hosta plants in a park in Harbin City in North-east China, and infection by HVX was confirmed by small RNA sequencing, RT-PCR and electron microscopy. The infectivity of HVX on Hosta ensata, a native species in North-east China, was confirmed by mechanical inoculation. We determined the complete genome sequence of an isolate of the virus, which revealed a high level of sequence similarity with other HVX genome sequences published to date. Phylogenetic analysis of HVX with other potexviruses based on the complete sequences revealed that all four HVX entries clustered to a single clade, which was most closely related to cassava common mosaic virus (CsCMV), hydrangea ringspot virus (HdRSV), plantago asiatica mosaic virus (PlAMV) and tulip virus X (TVX). Two potential recombination events were detected among the four HVX isolates. This work not only reports the complete genome sequence of an HVX isolate in North-east China for the first time, but also suggests the need to prevent further spread of this virus in the region.
KW - Potexvirus
KW - genome
KW - hosta virus X
KW - hostas
KW - small RNA sequencing
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U2 - 10.1080/07060661.2020.1736638
DO - 10.1080/07060661.2020.1736638
M3 - Comment/debate
AN - SCOPUS:85084975812
SN - 0706-0661
VL - 42
SP - 595
EP - 603
JO - Canadian Journal of Plant Pathology
JF - Canadian Journal of Plant Pathology
IS - 4
ER -