TY - JOUR
T1 - Odd-skipped related 2 splicing variants show opposite transcriptional activity
AU - Kawai, Shinji
AU - Kato, Takahiro
AU - Inaba, Hiroaki
AU - Okahashi, Nobuo
AU - Amano, Atsuo
N1 - Funding Information:
This study was supported in part by Grants-in-Aid for Scientific Research from the Japanese Society for the Promotion of Science (No. 15390645). This work was a part of the 21st Century COE entitled “Origination of Frontier BioDentistry” at Osaka University Graduate School of Dentistry, supported by the Ministry of Education, Culture, Sports, Science and Technology.
PY - 2005/3/4
Y1 - 2005/3/4
N2 - Odd-skipped related 2 (Osr2) is expressed in the domain of epithelial-mesenchymal interactions during tooth and kidney development. In this study, we report that alternative splicing of exon 4 resulted in two transcripts, Osr2A and Osr2B. These variants utilized a different splice acceptor, and Osr2B exhibited a frameshift followed by an early stop codon. Osr2A mRNA produced a protein that was 36 amino acids longer than Osr2B at the C-terminus and had five zinc-finger domains, whereas Osr2B had three zinc-finger motives. The 2 Osr2 variants were found in kidney, skeletal muscle, and testis, and Osr2B was predominantly transcribed in each. Flag-tagged variants showed the same localization in cell nuclei, however, Gal4 fusion proteins showed opposite transcriptional activities. Further, Flag-tagged variants also showed opposite transcriptional activities, though they were in contrast to the findings for the Gal4-fused variants. Our results indicate that Osr2 bifurcates its function by alternative splicing.
AB - Odd-skipped related 2 (Osr2) is expressed in the domain of epithelial-mesenchymal interactions during tooth and kidney development. In this study, we report that alternative splicing of exon 4 resulted in two transcripts, Osr2A and Osr2B. These variants utilized a different splice acceptor, and Osr2B exhibited a frameshift followed by an early stop codon. Osr2A mRNA produced a protein that was 36 amino acids longer than Osr2B at the C-terminus and had five zinc-finger domains, whereas Osr2B had three zinc-finger motives. The 2 Osr2 variants were found in kidney, skeletal muscle, and testis, and Osr2B was predominantly transcribed in each. Flag-tagged variants showed the same localization in cell nuclei, however, Gal4 fusion proteins showed opposite transcriptional activities. Further, Flag-tagged variants also showed opposite transcriptional activities, though they were in contrast to the findings for the Gal4-fused variants. Our results indicate that Osr2 bifurcates its function by alternative splicing.
KW - Alternative splicing
KW - Gene expression
KW - Gene structure
KW - Odd-skipped related 2
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U2 - 10.1016/j.bbrc.2004.12.173
DO - 10.1016/j.bbrc.2004.12.173
M3 - Article
C2 - 15670784
AN - SCOPUS:12844255841
SN - 0006-291X
VL - 328
SP - 306
EP - 311
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -