TY - JOUR
T1 - Oil accumulation by the oleaginous diatom Fistulifera solaris as revealed by the genome and transcriptome
AU - Tanaka, Tsuyoshi
AU - Maeda, Yoshiaki
AU - Veluchamy, Alaguraj
AU - Tanaka, Michihiro
AU - Abida, Heni
AU - Maréchal, Eric
AU - Bowler, Chris
AU - Muto, Masaki
AU - Sunaga, Yoshihiko
AU - Tanaka, Masayoshi
AU - Yoshino, Tomoko
AU - Taniguchi, Takeaki
AU - Fukuda, Yorikane
AU - Nemoto, Michiko
AU - Matsumoto, Mitsufumi
AU - Wong, Pui Shan
AU - Aburatani, Sachiyo
AU - Fujibuchi, Wataru
N1 - Publisher Copyright:
© 2015 American Society of Plant Biologists. All rights reserved.
PY - 2015
Y1 - 2015
N2 - Oleaginous photosynthetic organisms such as microalgae are promising sources for biofuel production through the generation of carbon-neutral sustainable energy. However, the metabolic mechanisms driving high-rate lipid production in these oleaginous organisms remain unclear, thus impeding efforts to improve productivity through genetic modifications. We analyzed the genome and transcriptome of the oleaginous diatom Fistulifera solaris JPCC DA0580. Next-generation sequencing technology provided evidence of an allodiploid genome structure, suggesting unorthodox molecular evolutionary and genetic regulatory systems for reinforcing metabolic efficiencies. Although major metabolic pathways were shared with nonoleaginous diatoms, transcriptome analysis revealed unique expression patterns, such as concomitant upregulation of fatty acid/triacylglycerol biosynthesis and fatty acid degradation (b-oxidation) in concert with ATP production. This peculiar pattern of gene expression may account for the simultaneous growth and oil accumulation phenotype and may inspire novel biofuel production technology based on this oleaginous microalga.
AB - Oleaginous photosynthetic organisms such as microalgae are promising sources for biofuel production through the generation of carbon-neutral sustainable energy. However, the metabolic mechanisms driving high-rate lipid production in these oleaginous organisms remain unclear, thus impeding efforts to improve productivity through genetic modifications. We analyzed the genome and transcriptome of the oleaginous diatom Fistulifera solaris JPCC DA0580. Next-generation sequencing technology provided evidence of an allodiploid genome structure, suggesting unorthodox molecular evolutionary and genetic regulatory systems for reinforcing metabolic efficiencies. Although major metabolic pathways were shared with nonoleaginous diatoms, transcriptome analysis revealed unique expression patterns, such as concomitant upregulation of fatty acid/triacylglycerol biosynthesis and fatty acid degradation (b-oxidation) in concert with ATP production. This peculiar pattern of gene expression may account for the simultaneous growth and oil accumulation phenotype and may inspire novel biofuel production technology based on this oleaginous microalga.
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U2 - 10.1105/tpc.114.135194
DO - 10.1105/tpc.114.135194
M3 - Article
C2 - 25634988
AN - SCOPUS:84923045771
SN - 1040-4651
VL - 27
SP - 162
EP - 176
JO - Plant Cell
JF - Plant Cell
IS - 1
ER -