On the preparation of indoxyl red from indican and some new characteristics

Jingyuan Song, Mizuki Kitamatsu, Koreyoshi Imamura, Hitoshi Ohmori, Kouji Watanabe, Kazuhiro Nakanishi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


An indole compound with a strong purple-red color was produced by boiling a solution of indican under acidic conditions and purified by chromatographies on DEAE-650S Toyopearl TSK-gel and silica-gel columns. The purple-red compound purified was identified as indoxyl red, on the basis of FAB Mass, 13C NMR, 1H NMR, UV-visible spectra, and IR spectra. Although indoxyl red was first synthesized by Seidel9 70 years ago, very little information has been available on its characteristics. We repot here that the compound was purple-red colored at acidic pH and green at pH 13, and showed antiproliferative and cytotoxic activities to the mouse B cell lymphoma cell line NSF202.

Original languageEnglish
Pages (from-to)627-629
Number of pages3
JournalBioorganic and Medicinal Chemistry Letters
Issue number3
Publication statusPublished - Feb 1 2013


  • Cytotoxic activity
  • Indican
  • Indirubin
  • Indoxyl
  • Mouse B cell lymphoma cell line NSF202

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry


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