Original: Influence of Three-Dimensional Scaffold on Bone Induction by KUSA/A1 Cells

Andrea Paola Rodriguez, Hidetsugu Tsujigiwa, Silvia Borkosky, Phuu Pwint Han, Ryo Tamamura, Mehmet Gunduz, Hitoshi Nagatsuka, Noriyuki Nagai, Liliana Missana

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Recently, atelocollagen has been used in tissue engineering as one of the most useful scaffolds for bone formation. However, the optimal scaffold-structure for bone engineering has not been determined. Hence, we compared the honeycomb porous structure (HPS) and the interconnected porous structure (IPS) with or without KUSA/A1 cells in vivo. KUSA/A1 cells alone, HPS alone, IPS alone, KUSA/A1-HPS and KUSA/A1-1PS were implanted in the subcutaneous pockets of 4-weeks-old male SCID mice. The implants were examined radiologically and histologically 1, 2,4 and 8 weeks after implantation. Radiologically, small radiopaque islands of new bone were observed in the implant of KUSA/A1 cells alone. No radiopacity was observed with both scaffols alone. In the KUSA/A 1 -HPS implant, the scaffold was partially filled with radiopaque areas. In contrast, in the KUSA/A1-IPS implant, the whole scaffold was filled with a well-defined radiopaque area. Histologically, KUSA/A1 cells alone induced only small nests of new bone. HPS alone produced inflammatory reaction. A large bone was observed in implants of KUSA/A1 cells combined with both scaffolds, but the scaffold was completely filled with new bone without inflammatory reaction within a shorter period in the KUSA/A1-IPS compared to KUSA/A1-HPS implant. Our results indicated that a cotton structured scaffold plays an important role in carrying the cells, providing the precise size, shape and comfortable environment. These results have potential impact to enhance existing therapeutic strategies.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalJournal of Hard Tissue Biology
Issue number2
Publication statusPublished - Jan 2004


  • KUSA/A1 cells
  • bone induction
  • histology
  • radiologic examination
  • scaffold

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biomaterials
  • Orthopedics and Sports Medicine
  • Dentistry(all)
  • Cell Biology


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