Oscillatory intracellular Ca2+ responses in living bone

Yoshihito Ishihara, Yasuyo Sugawara, Hiroshi Kamioka, Noriaki Kawanabe, Keiji Naruse, Takashi Yamashiro

Research output: Contribution to journalReview articlepeer-review

1 Citation (Scopus)


Intracellular calcium (Ca2+) is an important secondary messenger that modulates many cellular processes. Its oscillatory signaling is considered to participate in the regulation of many different cell functions, including bone metabolism. However, it is not entirely clear whether Ca2+ oscillations occur between osteoblasts and osteocytes in integrated bone tissues because of the complex mineralized matrices surrounding bone cells. To address this issue, we have recently developed a novel ex vivo realtime imaging system, which made it possible to observe repetitive and autonomous oscillations in the intracellular Ca2+ concentration ([Ca2+]i) in intact chick calvarial explants. This system revealed that Ca2+ release from intracellular stores plays a key role in Ca2+ oscillations in bone cells. Additionally, gap junctions are important for the maintenance of these oscillations in osteocytes but not in osteoblasts. In this review, we describe the dynamic oscillatory elevations of Ca2+ levels that occur in osteoblasts and osteocytes in living bone.

Original languageEnglish
Pages (from-to)49-53
Number of pages5
Journaljournal of oral biosciences
Issue number2
Publication statusPublished - May 2014


  • Calcium oscillation
  • Gap junction
  • Intracellular calcium stores
  • Osteoblast
  • Osteocyte

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Dentistry(all)


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