Osteoblast Response of Additively Manufactured Zirconia and Alumina-Toughened Zirconia

Hiroto Nakai, Masanao Inokoshi, Kosuke Nozaki, Kumiko Yoshihara, Akihiro Matsukawa, Noriyuki Nagaoka, Watcharapong Tonprasong, Shunsuke Minakuchi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Zirconia ceramics have been widely used in dentistry. Herein, we assess the surface morphology, surface texture, and osteoblast response of additively manufactured zirconia and alumina-toughened zirconia (ATZ) in comparison with titanium. The surface roughness, contact angle, and surface microstructure of titanium sandblasted with large-grit alumina and subsequently acid-etched using 18% HCl and 49% H2SO4 (SLA-titanium), uniaxially pressed zirconia (UP zirconia), additively manufactured zirconia (AM zirconia), and additively manufactured ATZ (AM ATZ) were investigated. Moreover, the cell viability, alkaline phosphatase (ALP) activity, and gene expression of type I collagen on these materials were evaluated. The data were statistically analyzed using one-way ANOVA with Tukey’s post hoc test. SLA-titanium showed the highest surface roughness and contact angle. The other three materials showed comparable surface roughness and contact angles. Micro- and nanoroughness were observed on the surface of SLA-titanium. UP zirconia and AM zirconia had similar surface morphologies. The cell viability, ALP activity, and gene expression of type I collagen on AM zirconia were comparable to or better than those on SLA-titanium. Our results indicate that AM zirconia is a promising material for zirconia dental implants.

Original languageEnglish
Article number8685
Issue number23
Publication statusPublished - Dec 2022


  • additive manufacturing
  • alumina-toughened zirconia
  • cell viability
  • osteogenic ability
  • zirconia

ASJC Scopus subject areas

  • General Materials Science
  • Condensed Matter Physics


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