Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo

Yoshinosuke Hamada; Kanako Yuki; Masayuki Okazaki; Wataru Fujitani; Takuya Matsumoto; Michiyo Kobashi Hashida; Kentaro Harutsugu; Kiyoshi Nokihara; Michiharu Daito; Nariaki Matsuura; Junzo Takahashi

doi:10.4012/dmj.23.650

Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo

Yoshinosuke Hamada, Kanako Yuki, Masayuki Okazaki, Wataru Fujitani, Takuya Matsumoto, Michiyo Kobashi Hashida, Kentaro Harutsugu, Kiyoshi Nokihara, Michiharu Daito, Nariaki Matsuura, Junzo Takahashi

Research output: Contribution to journal › Article › peer-review

59 Citations (Scopus)

Abstract

Our previous study reported that an osteopontin-derived peptide SVVYGLR activates the adhesion, migration and tube formation abilities of endothelial cells in vitro. The present study investigated angiogenesis due to synthetic SVVYGLR and mutant peptides in vivo. Mutant peptides (n = 7) were synthesized by substituting alanine (A) for one of the 7 amino acids comprising SVVYGLR. In dorsal air sac assay, mouse dorsal skin 5 days after implantation of a chamber filled with SVVYGLR had approximately the same number of newly formed blood vessels to that filled with vascular endothelial growth factor (VEGF). The ability of angiogenesis due to SVVAGLR was significantly lower than that due to other 6 mutant peptides and SVVYGLR. This indicates that tyrosine (Y) plays an important role in angiogenesis due to SVVYGLR.

Original language	English
Pages (from-to)	650-655
Number of pages	6
Journal	Dental materials journal
Volume	23
Issue number	4
DOIs	https://doi.org/10.4012/dmj.23.650
Publication status	Published - Dec 2004
Externally published	Yes

Keywords

Angiogenesis
Endothelial cells
SVVYGLR

ASJC Scopus subject areas

Ceramics and Composites
Dentistry(all)

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title = "Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo",

abstract = "Our previous study reported that an osteopontin-derived peptide SVVYGLR activates the adhesion, migration and tube formation abilities of endothelial cells in vitro. The present study investigated angiogenesis due to synthetic SVVYGLR and mutant peptides in vivo. Mutant peptides (n = 7) were synthesized by substituting alanine (A) for one of the 7 amino acids comprising SVVYGLR. In dorsal air sac assay, mouse dorsal skin 5 days after implantation of a chamber filled with SVVYGLR had approximately the same number of newly formed blood vessels to that filled with vascular endothelial growth factor (VEGF). The ability of angiogenesis due to SVVAGLR was significantly lower than that due to other 6 mutant peptides and SVVYGLR. This indicates that tyrosine (Y) plays an important role in angiogenesis due to SVVYGLR.",

keywords = "Angiogenesis, Endothelial cells, SVVYGLR",

author = "Yoshinosuke Hamada and Kanako Yuki and Masayuki Okazaki and Wataru Fujitani and Takuya Matsumoto and {Kobashi Hashida}, Michiyo and Kentaro Harutsugu and Kiyoshi Nokihara and Michiharu Daito and Nariaki Matsuura and Junzo Takahashi",

year = "2004",

month = dec,

doi = "10.4012/dmj.23.650",

language = "English",

volume = "23",

pages = "650--655",

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publisher = "Japanese Society for Dental Materials and Devices",

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T1 - Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo

AU - Hamada, Yoshinosuke

AU - Yuki, Kanako

AU - Okazaki, Masayuki

AU - Fujitani, Wataru

AU - Matsumoto, Takuya

AU - Kobashi Hashida, Michiyo

AU - Harutsugu, Kentaro

AU - Nokihara, Kiyoshi

AU - Daito, Michiharu

AU - Matsuura, Nariaki

AU - Takahashi, Junzo

PY - 2004/12

Y1 - 2004/12

N2 - Our previous study reported that an osteopontin-derived peptide SVVYGLR activates the adhesion, migration and tube formation abilities of endothelial cells in vitro. The present study investigated angiogenesis due to synthetic SVVYGLR and mutant peptides in vivo. Mutant peptides (n = 7) were synthesized by substituting alanine (A) for one of the 7 amino acids comprising SVVYGLR. In dorsal air sac assay, mouse dorsal skin 5 days after implantation of a chamber filled with SVVYGLR had approximately the same number of newly formed blood vessels to that filled with vascular endothelial growth factor (VEGF). The ability of angiogenesis due to SVVAGLR was significantly lower than that due to other 6 mutant peptides and SVVYGLR. This indicates that tyrosine (Y) plays an important role in angiogenesis due to SVVYGLR.

AB - Our previous study reported that an osteopontin-derived peptide SVVYGLR activates the adhesion, migration and tube formation abilities of endothelial cells in vitro. The present study investigated angiogenesis due to synthetic SVVYGLR and mutant peptides in vivo. Mutant peptides (n = 7) were synthesized by substituting alanine (A) for one of the 7 amino acids comprising SVVYGLR. In dorsal air sac assay, mouse dorsal skin 5 days after implantation of a chamber filled with SVVYGLR had approximately the same number of newly formed blood vessels to that filled with vascular endothelial growth factor (VEGF). The ability of angiogenesis due to SVVAGLR was significantly lower than that due to other 6 mutant peptides and SVVYGLR. This indicates that tyrosine (Y) plays an important role in angiogenesis due to SVVYGLR.

KW - Angiogenesis

KW - Endothelial cells

KW - SVVYGLR

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SN - 0287-4547

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EP - 655

JO - Dental materials journal

JF - Dental materials journal

IS - 4

ER -

Osteopontin-derived peptide SVVYGLR induces angiogenesis in vivo

Abstract

Keywords

ASJC Scopus subject areas

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