Over-expression of a Barley Aquaporin Increased the Shoot/Root Ratio and Raised Salt Sensitivity in Transgenic Rice Plants

Maki Katsuhara; Kazuki Koshio; Mineo Shibasaka; Yasuyuki Hayashi; Takahiko Hayakawa; Kunihiro Kasamo

doi:10.1093/pcp/pcg167

Over-expression of a Barley Aquaporin Increased the Shoot/Root Ratio and Raised Salt Sensitivity in Transgenic Rice Plants

Maki Katsuhara, Kazuki Koshio, Mineo Shibasaka, Yasuyuki Hayashi, Takahiko Hayakawa, Kunihiro Kasamo

Institute of Plant Science and Resources

Research output: Contribution to journal › Article › peer-review

160 Citations (Scopus)

Abstract

Barley HvPIP2;1 is a plasma membrane aquaporin and its expression was down-regulated after salt stress in barley [Katsuhara et al. (2002) Plant Cell Physiol. 43: 885]. We produced and analyzed transgenic rice plants over-expressing barley HvPIP2;1 in the present study. Over-expression of HvPIP2;1 increased (1) radial hydraulic conductivity of roots (Lp_r) to 140%, and (2) the mass ratio of shoot to root up to 150%. In these transgenic rice plants under salt stress of 100 mM NaCl, growth reduction was greater than in non-transgenic plants. A decrease in shoot water content (from 79% to 61%) and reduction of root mass or shoot mass (both less than 40% of non-stressed plants) were observed in transgenic plants under salt stress for 2 weeks. These results indicated that over-expression of HvPIP2;1 makes rice plants sensitive to 100 mM NaCl. The possible involvement of aquaporins in salt tolerance is discussed.

Original language	English
Pages (from-to)	1378-1383
Number of pages	6
Journal	Plant and Cell Physiology
Volume	44
Issue number	12
DOIs	https://doi.org/10.1093/pcp/pcg167
Publication status	Published - Dec 2003

Keywords

Aquaporin
Barley
Salt stress
Transgenic rice
Water transport

ASJC Scopus subject areas

Physiology
Plant Science
Cell Biology

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10.1093/pcp/pcg167

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title = "Over-expression of a Barley Aquaporin Increased the Shoot/Root Ratio and Raised Salt Sensitivity in Transgenic Rice Plants",

abstract = "Barley HvPIP2;1 is a plasma membrane aquaporin and its expression was down-regulated after salt stress in barley [Katsuhara et al. (2002) Plant Cell Physiol. 43: 885]. We produced and analyzed transgenic rice plants over-expressing barley HvPIP2;1 in the present study. Over-expression of HvPIP2;1 increased (1) radial hydraulic conductivity of roots (Lpr) to 140%, and (2) the mass ratio of shoot to root up to 150%. In these transgenic rice plants under salt stress of 100 mM NaCl, growth reduction was greater than in non-transgenic plants. A decrease in shoot water content (from 79% to 61%) and reduction of root mass or shoot mass (both less than 40% of non-stressed plants) were observed in transgenic plants under salt stress for 2 weeks. These results indicated that over-expression of HvPIP2;1 makes rice plants sensitive to 100 mM NaCl. The possible involvement of aquaporins in salt tolerance is discussed.",

keywords = "Aquaporin, Barley, Salt stress, Transgenic rice, Water transport",

author = "Maki Katsuhara and Kazuki Koshio and Mineo Shibasaka and Yasuyuki Hayashi and Takahiko Hayakawa and Kunihiro Kasamo",

note = "Funding Information: Most of this work was supported by the Bio Design Program (BDP), BDP-01-I-2-3. Research on the characteristics of the barely HvPIP2;1 gene was supported by CREST of JST (Japan Science and Technology Corporation). The transformation of rice was supported by the Research Association for Biotechnology (RAB) in a project entitled “CO2 fixation in arid areas using biological functions”.",

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doi = "10.1093/pcp/pcg167",

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AU - Katsuhara, Maki

AU - Koshio, Kazuki

AU - Shibasaka, Mineo

AU - Hayashi, Yasuyuki

AU - Hayakawa, Takahiko

AU - Kasamo, Kunihiro

N1 - Funding Information: Most of this work was supported by the Bio Design Program (BDP), BDP-01-I-2-3. Research on the characteristics of the barely HvPIP2;1 gene was supported by CREST of JST (Japan Science and Technology Corporation). The transformation of rice was supported by the Research Association for Biotechnology (RAB) in a project entitled “CO2 fixation in arid areas using biological functions”.

PY - 2003/12

Y1 - 2003/12

N2 - Barley HvPIP2;1 is a plasma membrane aquaporin and its expression was down-regulated after salt stress in barley [Katsuhara et al. (2002) Plant Cell Physiol. 43: 885]. We produced and analyzed transgenic rice plants over-expressing barley HvPIP2;1 in the present study. Over-expression of HvPIP2;1 increased (1) radial hydraulic conductivity of roots (Lpr) to 140%, and (2) the mass ratio of shoot to root up to 150%. In these transgenic rice plants under salt stress of 100 mM NaCl, growth reduction was greater than in non-transgenic plants. A decrease in shoot water content (from 79% to 61%) and reduction of root mass or shoot mass (both less than 40% of non-stressed plants) were observed in transgenic plants under salt stress for 2 weeks. These results indicated that over-expression of HvPIP2;1 makes rice plants sensitive to 100 mM NaCl. The possible involvement of aquaporins in salt tolerance is discussed.

AB - Barley HvPIP2;1 is a plasma membrane aquaporin and its expression was down-regulated after salt stress in barley [Katsuhara et al. (2002) Plant Cell Physiol. 43: 885]. We produced and analyzed transgenic rice plants over-expressing barley HvPIP2;1 in the present study. Over-expression of HvPIP2;1 increased (1) radial hydraulic conductivity of roots (Lpr) to 140%, and (2) the mass ratio of shoot to root up to 150%. In these transgenic rice plants under salt stress of 100 mM NaCl, growth reduction was greater than in non-transgenic plants. A decrease in shoot water content (from 79% to 61%) and reduction of root mass or shoot mass (both less than 40% of non-stressed plants) were observed in transgenic plants under salt stress for 2 weeks. These results indicated that over-expression of HvPIP2;1 makes rice plants sensitive to 100 mM NaCl. The possible involvement of aquaporins in salt tolerance is discussed.

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Over-expression of a Barley Aquaporin Increased the Shoot/Root Ratio and Raised Salt Sensitivity in Transgenic Rice Plants

Abstract

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