TY - JOUR
T1 - Overexpression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 decreases bone density in adult mice and induces dwarfism
AU - Nakanishi, Tohru
AU - Yamaai, Tomoichiro
AU - Asano, Masahiro
AU - Nawachi, Kumiko
AU - Suzuki, Misao
AU - Sugimoto, Tomosada
AU - Takigawa, Masaharu
N1 - Funding Information:
We thank Dr. Kenichi Yamamura (Center for Animal Resources and Development, Kumamoto University) for generating the Tg mice. We also thank Ms. Etsuko Fujisawa, Kazumi Ohyama, and Yuki Nonami for secretarial assistance. This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan (to T.N. and M.T.), grants from the Naito Foundation (to M.T.), the Sumitomo Foundation (to M.T.), and the Foundation for Growth Science in Japan (to M.T.), and the Research for the Future Programme of the Japanese Society for the Promotion of Science (JSPS) (Project: Biological Tissue Engineering, JSPS-RFTF98100201).
PY - 2001
Y1 - 2001
N2 - Connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24) is a multifunctional growth factor for fibroblasts, chondrocytes, and vascular endothelial cells. In the present study, we established transgenic (Tg) mice that over-produce CTGF/Hcs24 under the control of mouse type XI collagen promoter. Tg mice could develop and their embryonic and neonatal growth occurred normally. But they showed dwarfism within a few months of birth. X-ray analysis revealed that their bone density was decreased compared with normal mice. The femurs in the hindlimbs in particular showed an apparent low density. These results indicated that overexpression of CTGF/Hcs24 affects certain steps of endochondral ossification. In addition, the testes were much smaller than normal and fertility was affected in Tg mice, indicating that CTGF/Hcs24 may also regulate the embryonic development of the testis.
AB - Connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24) is a multifunctional growth factor for fibroblasts, chondrocytes, and vascular endothelial cells. In the present study, we established transgenic (Tg) mice that over-produce CTGF/Hcs24 under the control of mouse type XI collagen promoter. Tg mice could develop and their embryonic and neonatal growth occurred normally. But they showed dwarfism within a few months of birth. X-ray analysis revealed that their bone density was decreased compared with normal mice. The femurs in the hindlimbs in particular showed an apparent low density. These results indicated that overexpression of CTGF/Hcs24 affects certain steps of endochondral ossification. In addition, the testes were much smaller than normal and fertility was affected in Tg mice, indicating that CTGF/Hcs24 may also regulate the embryonic development of the testis.
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U2 - 10.1006/bbrc.2001.4379
DO - 10.1006/bbrc.2001.4379
M3 - Article
C2 - 11237711
AN - SCOPUS:0034817108
SN - 0006-291X
VL - 281
SP - 678
EP - 681
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -