Oxidative stress and altered antioxidant defenses in children with acute exacerbation of atopic dermatitis

Hirokazu Tsukahara, Rumiko Shibata, Yusei Ohshima, Yukiko Todoroki, Shuko Sato, Naoko Ohta, Masahiro Hiraoka, Akira Yoshida, Sankei Nishima, Mitsufumi Mayumi

Research output: Contribution to journalArticlepeer-review

149 Citations (Scopus)


The underlying mechanisms of skin inflammation in atopic dermatitis (AD) are not completely understood. The purpose of the present study was to examine the involvement of oxidative stress and antioxidant defenses in children with acute exacerbation of AD. We studied 13 children who were hospitalized for acute exacerbation of AD with purulent skin infection by Staphylococcal aureus (age, 1.5 to 10.0 years), and 28 age-matched healthy subjects (controls). Urine samples obtained from the patients on admission, on 2nd and 7th-9th hospital days, as well as from the controls were analyzed for 8-hydroxy-2′-deoxyguanosine (8-OHdG) (a marker of oxidative DNA damage), acrolein-lysine adducts (a marker of lipid peroxidation), bilirubin oxidative metabolites (BOM) (a marker of antioxidant activity of bilirubin under oxidative stress) and nitrite/nitrate (NOx-) (a marker of endogenous nitric oxide production). Of these, urinary concentrations of 8-OHdG, acrolein-lysine adducts and BOM, but not NOx-, were significantly higher in AD children on admission than those in control subjects. Response to treatment was associated with significant falls in the concentrations of 8-OHdG and acrolein-lysine adducts. Urinary concentrations of acrolein-lysine adducts, but not 8-OHdG, were still significantly higher in AD patients on the 7th-9th hospital day relative to the control. Urinary BOM remained almost constant and significantly high in AD children during hospitalization. Our findings indicate that oxidative stress and altered antioxidant defenses are involved in the pathophysiology of acute exacerbation of AD, and that suppression of oxidative stress might be a potentially useful strategy for the treatment of AD.

Original languageEnglish
Pages (from-to)2509-2516
Number of pages8
JournalLife Sciences
Issue number22
Publication statusPublished - Apr 18 2003
Externally publishedYes


  • 8-Hydroxy-2′-deoxyguanosine
  • Acrolein
  • Acute exacerbation
  • Atopic dermatitis
  • Bilirubin oxidative metabolites
  • Oxidative stress

ASJC Scopus subject areas

  • General Pharmacology, Toxicology and Pharmaceutics
  • General Biochemistry,Genetics and Molecular Biology


Dive into the research topics of 'Oxidative stress and altered antioxidant defenses in children with acute exacerbation of atopic dermatitis'. Together they form a unique fingerprint.

Cite this