Panose production by a mutant of Streptomyces hygroscopicus subsp. limoneus

Mohammad Mahfuzul Hoque, Toshinari Ohashi, Yoshinobu Kimura, Shuhei Nakajima, Koji Nagamine, Eiji Higashide

Research output: Contribution to journalArticlepeer-review


One of the mutant non-producers of validamycin from Streptomyces hygroscopicus subsp. limoneus JCM 4911, designated as strain GT 32, showed the accumulation of panose in the culture broth under the conditions for validamycin production by the original strain. Panose production was not detected in the culture broth of the original strain which was known to produce the antibiotic, validamycin, or in that of the other mutant strains studied. The structure of panose, O- α -D-glucopyranosyl-(1 → 6)-O- α -D-glucopyranosyl-(1 → 4)-O- α -D-glucopyranose, for the oligosaccharide specifically produced by the strain GT-32, was identified by sugar composition analysis, ion-spray mass spectrometry, methylation analysis, and 500 MHz 1H-NMR. The maximum production (1.59 mg/ml) of panose by the strain GT32 was found in 5 d cultured broth at 37°C on a rotary shaker at 180 rpm.

Original languageEnglish
Pages (from-to)7-12
Number of pages6
JournalBiocontrol science
Issue number1
Publication statusPublished - 1997


  • Ion-spray mass spectrometry
  • Panose production
  • Streptomyces hygroscopicus subsp. limoneus
  • Transglucosylation
  • Validamycin biosynthesis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Public Health, Environmental and Occupational Health


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