Parallel real-time PCR on a chip for genetic tug-of-war (gTOW) method

Toyohiro Naito; Ai Yatsuhashi; Noritada Kaji; Taeko Ando; Kazuo Sato; Hisao Moriya; Hiroaki Kitano; Takao Yasui; Manabu Tokeshi; Yoshinobu Baba

doi:10.2116/analsci.29.367

Parallel real-time PCR on a chip for genetic tug-of-war (gTOW) method

Toyohiro Naito, Ai Yatsuhashi, Noritada Kaji, Taeko Ando, Kazuo Sato, Hisao Moriya, Hiroaki Kitano, Takao Yasui, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

A microchip-based real-time polymerase chain reaction (PCR) device has been developed for the genetic tug-of-war (gTOW) method that provides quantitative data for research on biorobustness and systems biology. The device was constructed of a silicon glass chip, a temperature controlling Peltier element, and a microscope. A parallel real-time amplification process of target genes on the plasmids and the housekeeping genes in a model eukaryote Saccharomyces cerevisiae were detected simultaneously, and the copy number of the target genes were estimated. The device provides unique quantitative data that can be used to augment understanding of the system-level properties of living cells.

Original language	English
Pages (from-to)	367-371
Number of pages	5
Journal	Analytical Sciences
Volume	29
Issue number	3
DOIs	https://doi.org/10.2116/analsci.29.367
Publication status	Published - 2013

ASJC Scopus subject areas

Analytical Chemistry

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abstract = "A microchip-based real-time polymerase chain reaction (PCR) device has been developed for the genetic tug-of-war (gTOW) method that provides quantitative data for research on biorobustness and systems biology. The device was constructed of a silicon glass chip, a temperature controlling Peltier element, and a microscope. A parallel real-time amplification process of target genes on the plasmids and the housekeeping genes in a model eukaryote Saccharomyces cerevisiae were detected simultaneously, and the copy number of the target genes were estimated. The device provides unique quantitative data that can be used to augment understanding of the system-level properties of living cells.",

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AU - Naito, Toyohiro

AU - Yatsuhashi, Ai

AU - Kaji, Noritada

AU - Ando, Taeko

AU - Sato, Kazuo

AU - Moriya, Hisao

AU - Kitano, Hiroaki

AU - Yasui, Takao

AU - Tokeshi, Manabu

AU - Baba, Yoshinobu

PY - 2013

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Parallel real-time PCR on a chip for genetic tug-of-war (gTOW) method

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