TY - JOUR
T1 - Parasporin-2Ab, a newly isolated cytotoxic crystal protein from Bacillus thuringiensis
AU - Hayakawa, Tohru
AU - Kanagawa, Rie
AU - Kotani, Yosuke
AU - Kimura, Mayumi
AU - Yamagiwa, Masashi
AU - Yamane, Yoshiharu
AU - Takebe, So
AU - Sakai, Hiroshi
PY - 2007/10/1
Y1 - 2007/10/1
N2 - A novel crystal protein that exhibited potent cytotoxicity against human leukemic T-cells was cloned from the Bacillus thuringiensis TK-E6 strain. The protein, designated as parasporin-2Ab (PS2Ab), was a polypeptide of 304 amino acid residues with a predicted molecular weight of 33,017. The deduced amino acid sequence of PS2Ab showed significant homology (84% identitiy) to parasporin-2Aa (PS2Aa) from the B. thuringiensis A1547 strain. Upon processing of PS2Ab with proteinase K, the active form of 29 kDa was produced. The activated PS2Ab showed potent cytotoxicity against MOLT-4 and Jurkat cells and the EC50 values were estimated as 0.545 and 0.745 ng/mL, respectively. The cytotoxicity of PS2Ab was significantly higher than that of PS2Aa reported elsewhere. Although both cytotoxins were structurally related, it was thought that the minor differences found were responsible for the different cytotoxicities of PS2Ab and PS2Aa.
AB - A novel crystal protein that exhibited potent cytotoxicity against human leukemic T-cells was cloned from the Bacillus thuringiensis TK-E6 strain. The protein, designated as parasporin-2Ab (PS2Ab), was a polypeptide of 304 amino acid residues with a predicted molecular weight of 33,017. The deduced amino acid sequence of PS2Ab showed significant homology (84% identitiy) to parasporin-2Aa (PS2Aa) from the B. thuringiensis A1547 strain. Upon processing of PS2Ab with proteinase K, the active form of 29 kDa was produced. The activated PS2Ab showed potent cytotoxicity against MOLT-4 and Jurkat cells and the EC50 values were estimated as 0.545 and 0.745 ng/mL, respectively. The cytotoxicity of PS2Ab was significantly higher than that of PS2Aa reported elsewhere. Although both cytotoxins were structurally related, it was thought that the minor differences found were responsible for the different cytotoxicities of PS2Ab and PS2Aa.
UR - http://www.scopus.com/inward/record.url?scp=34648817546&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34648817546&partnerID=8YFLogxK
U2 - 10.1007/s00284-006-0351-8
DO - 10.1007/s00284-006-0351-8
M3 - Article
C2 - 17700988
AN - SCOPUS:34648817546
SN - 0343-8651
VL - 55
SP - 278
EP - 283
JO - Current Microbiology
JF - Current Microbiology
IS - 4
ER -