Participation of adult mouse bone marrow cells in reconstitution of skin

Ken Kataoka, Reinhold J. Medina, Tomofumi Kageyama, Masahiro Miyazaki, Tadashi Yoshino, Teruhiko Makino, Nam Ho Huh

Research output: Contribution to journalArticlepeer-review

121 Citations (Scopus)


Results of recent studies have indicated that bone marrow cells can differentiate into various cells of ectodermal, mesodermal, and endodermal origins when transplanted into the body. However, the problems associated with those experiments such as the long latent period, rareness of the event, and difficulty in controlling the processes have hampered detailed mechanistic studies. In the present study, we examined the potency of mouse bone marrow cells to differentiate into cells comprising skin tissues using a skin reconstitution assay. Bone marrow cells from adult green fluorescent protein (GFP)-transgenic mice were transplanted in a mixture of embryonic mouse skin cells (17.5 days post-coitus) onto skin defects made on the backs of nude mice. Within 3 weeks, fully differentiated skin with hair was reconstituted. GFP-positive cells were found in the epidermis, hair follicles, sebaceous glands, and dermis. The localization and morphology of the cells, results of immunohistochemistry, and results of specific staining confirmed that the bone marrow cells had differentiated into epidermal keratinocytes, sebaceous gland cells, follicular epithelial cells, dendritic cells, and endothelial cells under the present conditions. These results indicate that this system is suitable for molecular and cellular mechanistic studies on differentiation of stem cells to various epidermal and dermal cells.

Original languageEnglish
Pages (from-to)1227-1231
Number of pages5
JournalAmerican Journal of Pathology
Issue number4
Publication statusPublished - Oct 1 2003

ASJC Scopus subject areas

  • Pathology and Forensic Medicine


Dive into the research topics of 'Participation of adult mouse bone marrow cells in reconstitution of skin'. Together they form a unique fingerprint.

Cite this