Participation of cathepsin L on bone resorption

Hisao Kakegawa; Takeshi Nikawa; Kahori Tagami; Hiroshi Kamioka; Koji Sumitani; Terushige Kawata; Marinka Drobnič-Kosorok; Brigita Lenarčič; Vito Turk; Nobuhiko Katunuma

doi:10.1016/0014-5793(93)80118-E

Participation of cathepsin L on bone resorption

Hisao Kakegawa, Takeshi Nikawa, Kahori Tagami, Hiroshi Kamioka, Koji Sumitani, Terushige Kawata, Marinka Drobnič-Kosorok, Brigita Lenarčič, Vito Turk, Nobuhiko Katunuma

Research output: Contribution to journal › Article › peer-review

93 Citations (Scopus)

Abstract

The proteinase responsible for bone collagen degradation in osteo-resorption was examined. The bone pit formation induced by parathyroid hormone (PTH) was markedly suppressed by leupeptin, E-64 and cystatin A, while no inhibition was observed by CA-074, a specific inhibitor of cathepsin B. Pig leucocyte cysteine proteinase inhibitor (PLCPI), a specific inhibitor of cathepsin L, and chymostatin, a selective inhibitor of cathepsin L, completely inhibited the pit formation. Cathepsin L activity in osteoclasts was much higher than the other cathepsin activities. Serum calcium in rats placed on a low calcium diet was decreased by treatment of E-64 or cystatin A, but not by CA-074. These findings suggest that cathepsin L is the main proteinase responsible for bone collagen degradation.

Original language	English
Pages (from-to)	247-250
Number of pages	4
Journal	FEBS Letters
Volume	321
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(93)80118-E
Publication status	Published - Apr 26 1993
Externally published	Yes

Keywords

Bone resorption
CA-074
Cathepsin L
Cystatin A
E-64
Pig leucocyte cysteine proteinase inhibitor

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(93)80118-E

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title = "Participation of cathepsin L on bone resorption",

abstract = "The proteinase responsible for bone collagen degradation in osteo-resorption was examined. The bone pit formation induced by parathyroid hormone (PTH) was markedly suppressed by leupeptin, E-64 and cystatin A, while no inhibition was observed by CA-074, a specific inhibitor of cathepsin B. Pig leucocyte cysteine proteinase inhibitor (PLCPI), a specific inhibitor of cathepsin L, and chymostatin, a selective inhibitor of cathepsin L, completely inhibited the pit formation. Cathepsin L activity in osteoclasts was much higher than the other cathepsin activities. Serum calcium in rats placed on a low calcium diet was decreased by treatment of E-64 or cystatin A, but not by CA-074. These findings suggest that cathepsin L is the main proteinase responsible for bone collagen degradation.",

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author = "Hisao Kakegawa and Takeshi Nikawa and Kahori Tagami and Hiroshi Kamioka and Koji Sumitani and Terushige Kawata and Marinka Drobni{\v c}-Kosorok and Brigita Lenar{\v c}i{\v c} and Vito Turk and Nobuhiko Katunuma",

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day = "26",

doi = "10.1016/0014-5793(93)80118-E",

language = "English",

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T1 - Participation of cathepsin L on bone resorption

AU - Kakegawa, Hisao

AU - Nikawa, Takeshi

AU - Tagami, Kahori

AU - Kamioka, Hiroshi

AU - Sumitani, Koji

AU - Kawata, Terushige

AU - Drobnič-Kosorok, Marinka

AU - Lenarčič, Brigita

AU - Turk, Vito

AU - Katunuma, Nobuhiko

PY - 1993/4/26

Y1 - 1993/4/26

N2 - The proteinase responsible for bone collagen degradation in osteo-resorption was examined. The bone pit formation induced by parathyroid hormone (PTH) was markedly suppressed by leupeptin, E-64 and cystatin A, while no inhibition was observed by CA-074, a specific inhibitor of cathepsin B. Pig leucocyte cysteine proteinase inhibitor (PLCPI), a specific inhibitor of cathepsin L, and chymostatin, a selective inhibitor of cathepsin L, completely inhibited the pit formation. Cathepsin L activity in osteoclasts was much higher than the other cathepsin activities. Serum calcium in rats placed on a low calcium diet was decreased by treatment of E-64 or cystatin A, but not by CA-074. These findings suggest that cathepsin L is the main proteinase responsible for bone collagen degradation.

AB - The proteinase responsible for bone collagen degradation in osteo-resorption was examined. The bone pit formation induced by parathyroid hormone (PTH) was markedly suppressed by leupeptin, E-64 and cystatin A, while no inhibition was observed by CA-074, a specific inhibitor of cathepsin B. Pig leucocyte cysteine proteinase inhibitor (PLCPI), a specific inhibitor of cathepsin L, and chymostatin, a selective inhibitor of cathepsin L, completely inhibited the pit formation. Cathepsin L activity in osteoclasts was much higher than the other cathepsin activities. Serum calcium in rats placed on a low calcium diet was decreased by treatment of E-64 or cystatin A, but not by CA-074. These findings suggest that cathepsin L is the main proteinase responsible for bone collagen degradation.

KW - Bone resorption

KW - CA-074

KW - Cathepsin L

KW - Cystatin A

KW - E-64

KW - Pig leucocyte cysteine proteinase inhibitor

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U2 - 10.1016/0014-5793(93)80118-E

DO - 10.1016/0014-5793(93)80118-E

M3 - Article

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AN - SCOPUS:0027509798

SN - 0014-5793

VL - 321

SP - 247

EP - 250

JO - FEBS Letters

JF - FEBS Letters

IS - 2-3

ER -

Participation of cathepsin L on bone resorption

Abstract

Keywords

ASJC Scopus subject areas

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