TY - JOUR
T1 - Participation of the hup gene product in site-specific DNA inversion in Escherichia coli
AU - Wada, Morimasa
AU - Kutsukake, Kazuhiro
AU - Komano, Teruya
AU - Imamoto, Fumio
AU - Kano, Yasunobu
PY - 1989/3/30
Y1 - 1989/3/30
N2 - The closely related Escherichia coli genes hupA and hupB each encode a bacterial histone-like protein HU. We report here that DNA inversion mediated by hin, gin, pin and rci but not by cin is blocked in a hupA hupB double mutant, although inversions in these systems occur in the hupA or hupB single mutant as efficiently as in the wild-type strain. These findings show that HU protein participates in site-specific DNA inversion in E. coli and that only one subunit, either HU-1 or HU-2, is sufficient for this inversion.
AB - The closely related Escherichia coli genes hupA and hupB each encode a bacterial histone-like protein HU. We report here that DNA inversion mediated by hin, gin, pin and rci but not by cin is blocked in a hupA hupB double mutant, although inversions in these systems occur in the hupA or hupB single mutant as efficiently as in the wild-type strain. These findings show that HU protein participates in site-specific DNA inversion in E. coli and that only one subunit, either HU-1 or HU-2, is sufficient for this inversion.
KW - HU protein
KW - Histone-like protein
KW - cin, gin, hin, pin systems
KW - hupA hupB mutant
KW - recombinant DNA
KW - recombinase
KW - shufflon
UR - http://www.scopus.com/inward/record.url?scp=0024558233&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024558233&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(89)90174-1
DO - 10.1016/0378-1119(89)90174-1
M3 - Article
C2 - 2666260
AN - SCOPUS:0024558233
SN - 0378-1119
VL - 76
SP - 345
EP - 352
JO - Gene
JF - Gene
IS - 2
ER -