Passive oral immunization by egg yolk immunoglobulin (IgY) to vibrio cholerae effectively prevents cholera

Kazuyuki Hirai; Hideyuki Arimitsu; Koji Umeda; Kenji Yokota; Lianhu Shen; Kiyoshi Ayada; Yoshikatsu Kodama; Takao Tsuji; Yoshikazu Hirai; Keiji Oguma

Passive oral immunization by egg yolk immunoglobulin (IgY) to vibrio cholerae effectively prevents cholera

Kazuyuki Hirai, Hideyuki Arimitsu, Koji Umeda, Kenji Yokota, Lianhu Shen, Kiyoshi Ayada, Yoshikatsu Kodama, Takao Tsuji, Yoshikazu Hirai, Keiji Oguma

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

In an attempt to prepare egg yolk immunoglobulin (IgY) to treat and prevent cholera, hens were immunized by a mixture of heat- or formalin-killed Vibrio cholerae O1 and O139 organisms, or by the recombinant cholera toxin B subunit (CTB). The IgYs were partially purified from egg yolk and orally administered to suckling mice before or after challenge with live O1 or O139 cells. The anti-O1 and O139 IgYs and the mixture of either IgY with anti-CTB IgY significantly protected the occurrence of cholera caused by both O1 and O139 infection. Since large amounts of IgY can be prepared very easily and at low cost, this seems to be a useful procedure for preventing and treating cholera.

Original language	English
Pages (from-to)	163-170
Number of pages	8
Journal	Acta medica Okayama
Volume	64
Issue number	3
Publication status	Published - Jul 2010

Keywords

IgY
O1
O139
Vibrio cholerae

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Passive oral immunization by egg yolk immunoglobulin (IgY) to vibrio cholerae effectively prevents cholera",

abstract = "In an attempt to prepare egg yolk immunoglobulin (IgY) to treat and prevent cholera, hens were immunized by a mixture of heat- or formalin-killed Vibrio cholerae O1 and O139 organisms, or by the recombinant cholera toxin B subunit (CTB). The IgYs were partially purified from egg yolk and orally administered to suckling mice before or after challenge with live O1 or O139 cells. The anti-O1 and O139 IgYs and the mixture of either IgY with anti-CTB IgY significantly protected the occurrence of cholera caused by both O1 and O139 infection. Since large amounts of IgY can be prepared very easily and at low cost, this seems to be a useful procedure for preventing and treating cholera.",

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author = "Kazuyuki Hirai and Hideyuki Arimitsu and Koji Umeda and Kenji Yokota and Lianhu Shen and Kiyoshi Ayada and Yoshikatsu Kodama and Takao Tsuji and Yoshikazu Hirai and Keiji Oguma",

year = "2010",

month = jul,

language = "English",

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pages = "163--170",

journal = "Acta medica Okayama",

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TY - JOUR

T1 - Passive oral immunization by egg yolk immunoglobulin (IgY) to vibrio cholerae effectively prevents cholera

AU - Hirai, Kazuyuki

AU - Arimitsu, Hideyuki

AU - Umeda, Koji

AU - Yokota, Kenji

AU - Shen, Lianhu

AU - Ayada, Kiyoshi

AU - Kodama, Yoshikatsu

AU - Tsuji, Takao

AU - Hirai, Yoshikazu

AU - Oguma, Keiji

PY - 2010/7

Y1 - 2010/7

N2 - In an attempt to prepare egg yolk immunoglobulin (IgY) to treat and prevent cholera, hens were immunized by a mixture of heat- or formalin-killed Vibrio cholerae O1 and O139 organisms, or by the recombinant cholera toxin B subunit (CTB). The IgYs were partially purified from egg yolk and orally administered to suckling mice before or after challenge with live O1 or O139 cells. The anti-O1 and O139 IgYs and the mixture of either IgY with anti-CTB IgY significantly protected the occurrence of cholera caused by both O1 and O139 infection. Since large amounts of IgY can be prepared very easily and at low cost, this seems to be a useful procedure for preventing and treating cholera.

AB - In an attempt to prepare egg yolk immunoglobulin (IgY) to treat and prevent cholera, hens were immunized by a mixture of heat- or formalin-killed Vibrio cholerae O1 and O139 organisms, or by the recombinant cholera toxin B subunit (CTB). The IgYs were partially purified from egg yolk and orally administered to suckling mice before or after challenge with live O1 or O139 cells. The anti-O1 and O139 IgYs and the mixture of either IgY with anti-CTB IgY significantly protected the occurrence of cholera caused by both O1 and O139 infection. Since large amounts of IgY can be prepared very easily and at low cost, this seems to be a useful procedure for preventing and treating cholera.

KW - IgY

KW - O1

KW - O139

KW - Vibrio cholerae

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UR - http://www.scopus.com/inward/citedby.url?scp=77954705125&partnerID=8YFLogxK

M3 - Article

C2 - 20596127

AN - SCOPUS:77954705125

SN - 0386-300X

VL - 64

SP - 163

EP - 170

JO - Acta medica Okayama

JF - Acta medica Okayama

IS - 3

ER -

Passive oral immunization by egg yolk immunoglobulin (IgY) to vibrio cholerae effectively prevents cholera

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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