Pathological examination of experimentally induced periodontal polyp in mice

Saeka Matsuda, Yukiko Yokoi, Keita Moriyama, Masahito Shoumura, Naoto Osuga, Keisuke Nakano, Toshiyuki Kawakami

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


The mechanism in the formation of periodontal polyp has been established in several histological studies but details on cell differentiation and/or proliferation have not been elucidated. In the present study, we established a convenient and possible experimental system using ddY mice. Briefly, pentobarbital sodium (Somnopentyl) was injected into the abdominal cavity of the mouse followed by access cavity preparation on maxillary first molar using low speed ½ round bur (Merufa Inc), exposing the pulp and then allowed to perforate the floor of the pulp chamber. Observation was done over time until 6 months using micro CT (m_CT) image photography. Results with transmission image using m_CT showed the expansion in the width of the periodontal ligament in the furcation area. The lesion was excised as one mass and examined histopathologically. The granulation tissue was covered with stratified squamous epithelium. The present experimental technique has been confirmed to be effective in analyzing the formation of periodontal polyp induced by mechanical perforation.

Original languageEnglish
Pages (from-to)397-400
Number of pages4
JournalJournal of Hard Tissue Biology
Issue number4
Publication statusPublished - Oct 23 2015


  • Experimental model
  • Histopathology
  • Micro-CT
  • Periodontal polyp

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biomaterials
  • Orthopedics and Sports Medicine
  • Dentistry(all)
  • Cell Biology


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