Pcf1, a large subunit of CAF-1, required for maintenance of checkpoint kinase Cds1 activity

Tatsuki Kunoh; Toshiyuki Habu

doi:10.1186/2193-1801-3-30

Pcf1, a large subunit of CAF-1, required for maintenance of checkpoint kinase Cds1 activity

Tatsuki Kunoh, Toshiyuki Habu

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Highly conserved chromatin assembly factor 1 (CAF-1) is required for histone deposition onto newly synthesized DNA to maintain genome stability. This study shows that the fission yeast Pcf1, the large subunit in CAF-1, is crucial for maintaining checkpoint kinase Cds1. Chromatin recruitment of Cds1 is enhanced by Pcf1 overproduction but is attenuated by the Δpcf1 mutation. Mutation of acetylation sites in the histone H4 tail abrogates the chromatin recruitment of Pcf1, which resembles that of Cds1 as reported previously. The present results provide evidence that chromatin recruitment of Pcf1, moderated by Clr6-HDAC activity, is essential for inactivating Cds1.

Original language	English
Article number	30
Pages (from-to)	1-8
Number of pages	8
Journal	SpringerPlus
Volume	3
Issue number	1
DOIs	https://doi.org/10.1186/2193-1801-3-30
Publication status	Published - 2014
Externally published	Yes

Keywords

Cds1
Chromatin
Clr6-HDAC
Fission yeast
Histone H4
Pcf/CAF-1

ASJC Scopus subject areas

General

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10.1186/2193-1801-3-30

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title = "Pcf1, a large subunit of CAF-1, required for maintenance of checkpoint kinase Cds1 activity",

abstract = "Highly conserved chromatin assembly factor 1 (CAF-1) is required for histone deposition onto newly synthesized DNA to maintain genome stability. This study shows that the fission yeast Pcf1, the large subunit in CAF-1, is crucial for maintaining checkpoint kinase Cds1. Chromatin recruitment of Cds1 is enhanced by Pcf1 overproduction but is attenuated by the Δpcf1 mutation. Mutation of acetylation sites in the histone H4 tail abrogates the chromatin recruitment of Pcf1, which resembles that of Cds1 as reported previously. The present results provide evidence that chromatin recruitment of Pcf1, moderated by Clr6-HDAC activity, is essential for inactivating Cds1.",

keywords = "Cds1, Chromatin, Clr6-HDAC, Fission yeast, Histone H4, Pcf/CAF-1",

author = "Tatsuki Kunoh and Toshiyuki Habu",

note = "Funding Information: We are particularly grateful to Drs. T. Matsumoto, M. Yanagida, K. Ekwall and R. C. Allshire for plasmids and strains. We thank Drs. K. Tanaka, J. Nakayama, M. Sadaie, T. Miyoshi and H. Kunoh for helpful discussion and procedures. We also acknowledge Dr. B. E. Hazen for reviewing and editing the manuscript. This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan to TH.",

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doi = "10.1186/2193-1801-3-30",

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T1 - Pcf1, a large subunit of CAF-1, required for maintenance of checkpoint kinase Cds1 activity

AU - Kunoh, Tatsuki

AU - Habu, Toshiyuki

N1 - Funding Information: We are particularly grateful to Drs. T. Matsumoto, M. Yanagida, K. Ekwall and R. C. Allshire for plasmids and strains. We thank Drs. K. Tanaka, J. Nakayama, M. Sadaie, T. Miyoshi and H. Kunoh for helpful discussion and procedures. We also acknowledge Dr. B. E. Hazen for reviewing and editing the manuscript. This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan to TH.

PY - 2014

Y1 - 2014

N2 - Highly conserved chromatin assembly factor 1 (CAF-1) is required for histone deposition onto newly synthesized DNA to maintain genome stability. This study shows that the fission yeast Pcf1, the large subunit in CAF-1, is crucial for maintaining checkpoint kinase Cds1. Chromatin recruitment of Cds1 is enhanced by Pcf1 overproduction but is attenuated by the Δpcf1 mutation. Mutation of acetylation sites in the histone H4 tail abrogates the chromatin recruitment of Pcf1, which resembles that of Cds1 as reported previously. The present results provide evidence that chromatin recruitment of Pcf1, moderated by Clr6-HDAC activity, is essential for inactivating Cds1.

AB - Highly conserved chromatin assembly factor 1 (CAF-1) is required for histone deposition onto newly synthesized DNA to maintain genome stability. This study shows that the fission yeast Pcf1, the large subunit in CAF-1, is crucial for maintaining checkpoint kinase Cds1. Chromatin recruitment of Cds1 is enhanced by Pcf1 overproduction but is attenuated by the Δpcf1 mutation. Mutation of acetylation sites in the histone H4 tail abrogates the chromatin recruitment of Pcf1, which resembles that of Cds1 as reported previously. The present results provide evidence that chromatin recruitment of Pcf1, moderated by Clr6-HDAC activity, is essential for inactivating Cds1.

KW - Cds1

KW - Chromatin

KW - Clr6-HDAC

KW - Fission yeast

KW - Histone H4

KW - Pcf/CAF-1

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ER -

Pcf1, a large subunit of CAF-1, required for maintenance of checkpoint kinase Cds1 activity

Abstract

Keywords

ASJC Scopus subject areas

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