Performance of protease as a cleaning agent for stainless steel surfaces fouled with protein

Takaharu Sakiyama, Toshihisa Toyomasu, Asuka Nagata, Koreyoshi Imamura, Tokio Takahashi, Tadashi Nagai, Kazuhiro Nakanishi

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Cleaning experiments were performed by feeding protease solutions as cleaning agents into a packed column of stainless steel particles fouled with β-lactoglobulin or gelatin. By evaluating the initial desorption rate constants (first-order rate constants) and residual amounts of protein, the cleaning efficiencies of the proteases were compared. The initial desorption rate constant depended on the kind of protease used and also on the type of protein to be removed. It increased with protease concentration and reached a constant value at a limit concentration. Kinetic analysis of the proteolytic reactions catalyzed by the proteases revealed that protease with a large Vmax/EKm value for the proteolytic reaction resulted in a large value of the initial desorption rate constant in the enzymatic cleaning at a low protease concentration. The Km value affected the limit concentration of protease yielding a saturation of initial desorption rate constant.

Original languageEnglish
Pages (from-to)297-301
Number of pages5
JournalJournal of Fermentation and Bioengineering
Issue number3
Publication statusPublished - 1998


  • Enzymatic cleaning
  • Gelatin
  • Protease
  • Stainless steel
  • β-lactoglobulin

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


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