Peroxidase-like catalytic activity of aqueous- and immobilized-Mn 3+-octabromo-porphyrins on ion-exchange resin supplied as mimetic of horseradish peroxidase

Youji Kitamura; Katsuya Mori; Makiko Yamamoto; Akira Nozaki; Madoka Saito; Ikuko Tsukamoto; Masaki Mifune; Yutaka Saito

doi:10.1248/cpb.56.1364

Peroxidase-like catalytic activity of aqueous- and immobilized-Mn ³⁺-octabromo-porphyrins on ion-exchange resin supplied as mimetic of horseradish peroxidase

Youji Kitamura, Katsuya Mori, Makiko Yamamoto, Akira Nozaki, Madoka Saito, Ikuko Tsukamoto, Masaki Mifune, Yutaka Saito

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

In order to explore the capability of metal porphyrins as an alternative of horseradish peroxidase (HRP), HRP-like activity of three manganese-porphyrins (Mn-Ps) and three Mn-octabromo-porphyrins (Mn-OBPs) was examined in both aqueous and immobilized states. It was found that Mn³⁺ octabromotetrakis (1-methyl-pyridinium-4yl)porphine (Mn-OBTMPyP) has an activity of at least 90% of HRP in an aqueous solution. Mn-OBTMPyP exhibited a catalytic activity even in the presence of hydrogen peroxide without suicide reaction. In addition, Mn-OBTMPyP was revealed to function as an alternative to HRP in the quantitative determination of serum uric acid. These results are of great interest because they indicate that metal-octabromo-porphyrins possibly include promising candidates of artificial enzyme capable of substituting for HRP.

Original language	English
Pages (from-to)	1364-1366
Number of pages	3
Journal	Chemical and Pharmaceutical Bulletin
Volume	56
Issue number	9
DOIs	https://doi.org/10.1248/cpb.56.1364
Publication status	Published - Sept 2008

Keywords

Bromination
Horseradish-peroxidase
Manganese-porphyrin
Mimetic

ASJC Scopus subject areas

Chemistry(all)
Drug Discovery

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1248/cpb.56.1364

Cite this

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title = "Peroxidase-like catalytic activity of aqueous- and immobilized-Mn 3+-octabromo-porphyrins on ion-exchange resin supplied as mimetic of horseradish peroxidase",

abstract = "In order to explore the capability of metal porphyrins as an alternative of horseradish peroxidase (HRP), HRP-like activity of three manganese-porphyrins (Mn-Ps) and three Mn-octabromo-porphyrins (Mn-OBPs) was examined in both aqueous and immobilized states. It was found that Mn3+ octabromotetrakis (1-methyl-pyridinium-4yl)porphine (Mn-OBTMPyP) has an activity of at least 90% of HRP in an aqueous solution. Mn-OBTMPyP exhibited a catalytic activity even in the presence of hydrogen peroxide without suicide reaction. In addition, Mn-OBTMPyP was revealed to function as an alternative to HRP in the quantitative determination of serum uric acid. These results are of great interest because they indicate that metal-octabromo-porphyrins possibly include promising candidates of artificial enzyme capable of substituting for HRP.",

keywords = "Bromination, Horseradish-peroxidase, Manganese-porphyrin, Mimetic",

author = "Youji Kitamura and Katsuya Mori and Makiko Yamamoto and Akira Nozaki and Madoka Saito and Ikuko Tsukamoto and Masaki Mifune and Yutaka Saito",

year = "2008",

month = sep,

doi = "10.1248/cpb.56.1364",

language = "English",

volume = "56",

pages = "1364--1366",

journal = "Chemical and Pharmaceutical Bulletin",

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publisher = "Pharmaceutical Society of Japan",

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T1 - Peroxidase-like catalytic activity of aqueous- and immobilized-Mn 3+-octabromo-porphyrins on ion-exchange resin supplied as mimetic of horseradish peroxidase

AU - Kitamura, Youji

AU - Mori, Katsuya

AU - Yamamoto, Makiko

AU - Nozaki, Akira

AU - Saito, Madoka

AU - Tsukamoto, Ikuko

AU - Mifune, Masaki

AU - Saito, Yutaka

PY - 2008/9

Y1 - 2008/9

N2 - In order to explore the capability of metal porphyrins as an alternative of horseradish peroxidase (HRP), HRP-like activity of three manganese-porphyrins (Mn-Ps) and three Mn-octabromo-porphyrins (Mn-OBPs) was examined in both aqueous and immobilized states. It was found that Mn3+ octabromotetrakis (1-methyl-pyridinium-4yl)porphine (Mn-OBTMPyP) has an activity of at least 90% of HRP in an aqueous solution. Mn-OBTMPyP exhibited a catalytic activity even in the presence of hydrogen peroxide without suicide reaction. In addition, Mn-OBTMPyP was revealed to function as an alternative to HRP in the quantitative determination of serum uric acid. These results are of great interest because they indicate that metal-octabromo-porphyrins possibly include promising candidates of artificial enzyme capable of substituting for HRP.

AB - In order to explore the capability of metal porphyrins as an alternative of horseradish peroxidase (HRP), HRP-like activity of three manganese-porphyrins (Mn-Ps) and three Mn-octabromo-porphyrins (Mn-OBPs) was examined in both aqueous and immobilized states. It was found that Mn3+ octabromotetrakis (1-methyl-pyridinium-4yl)porphine (Mn-OBTMPyP) has an activity of at least 90% of HRP in an aqueous solution. Mn-OBTMPyP exhibited a catalytic activity even in the presence of hydrogen peroxide without suicide reaction. In addition, Mn-OBTMPyP was revealed to function as an alternative to HRP in the quantitative determination of serum uric acid. These results are of great interest because they indicate that metal-octabromo-porphyrins possibly include promising candidates of artificial enzyme capable of substituting for HRP.

KW - Bromination

KW - Horseradish-peroxidase

KW - Manganese-porphyrin

KW - Mimetic

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