Phased A-tracts bind to the α subunit of RNA polymerase with increased affinity at low temperature

Seiichi Katayama; Osamu Matsushita; Eiji Tamai; Shigeru Miyata; Akinobu Okabe

doi:10.1016/S0014-5793(01)03148-9

Phased A-tracts bind to the α subunit of RNA polymerase with increased affinity at low temperature

Seiichi Katayama, Osamu Matsushita, Eiji Tamai, Shigeru Miyata, Akinobu Okabe

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Previously we showed that the expression of a Clostridium perfringens phospholipase C gene (plc) is activated by promoter upstream phased A-tracts in a low temperature-dependent manner. In this paper we characterize the interaction between the α subunit of C. perfringens RNA polymerase and the phased A-tracts. Hydroxyl radical footprinting and fluorescence polarization assaying revealed that the α subunit binds to the minor grooves of the phased A-tracts through its C-terminal domain with increased affinity at low temperature. The result provides a molecular mechanism underlying the activation of the plc promoter by the phased A-tracts.

Original language	English
Pages (from-to)	235-238
Number of pages	4
Journal	FEBS Letters
Volume	509
Issue number	2
DOIs	https://doi.org/10.1016/S0014-5793(01)03148-9
Publication status	Published - Dec 7 2001
Externally published	Yes

Keywords

Clostridium perfringens
Curved DNA
Gene expression
Phospholipase C
RNA polymerase
α Subunit

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(01)03148-9

Cite this

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title = "Phased A-tracts bind to the α subunit of RNA polymerase with increased affinity at low temperature",

abstract = "Previously we showed that the expression of a Clostridium perfringens phospholipase C gene (plc) is activated by promoter upstream phased A-tracts in a low temperature-dependent manner. In this paper we characterize the interaction between the α subunit of C. perfringens RNA polymerase and the phased A-tracts. Hydroxyl radical footprinting and fluorescence polarization assaying revealed that the α subunit binds to the minor grooves of the phased A-tracts through its C-terminal domain with increased affinity at low temperature. The result provides a molecular mechanism underlying the activation of the plc promoter by the phased A-tracts.",

keywords = "Clostridium perfringens, Curved DNA, Gene expression, Phospholipase C, RNA polymerase, α Subunit",

author = "Seiichi Katayama and Osamu Matsushita and Eiji Tamai and Shigeru Miyata and Akinobu Okabe",

note = "Funding Information: This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture, Japan.",

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doi = "10.1016/S0014-5793(01)03148-9",

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TY - JOUR

T1 - Phased A-tracts bind to the α subunit of RNA polymerase with increased affinity at low temperature

AU - Katayama, Seiichi

AU - Matsushita, Osamu

AU - Tamai, Eiji

AU - Miyata, Shigeru

AU - Okabe, Akinobu

N1 - Funding Information: This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture, Japan.

PY - 2001/12/7

Y1 - 2001/12/7

N2 - Previously we showed that the expression of a Clostridium perfringens phospholipase C gene (plc) is activated by promoter upstream phased A-tracts in a low temperature-dependent manner. In this paper we characterize the interaction between the α subunit of C. perfringens RNA polymerase and the phased A-tracts. Hydroxyl radical footprinting and fluorescence polarization assaying revealed that the α subunit binds to the minor grooves of the phased A-tracts through its C-terminal domain with increased affinity at low temperature. The result provides a molecular mechanism underlying the activation of the plc promoter by the phased A-tracts.

AB - Previously we showed that the expression of a Clostridium perfringens phospholipase C gene (plc) is activated by promoter upstream phased A-tracts in a low temperature-dependent manner. In this paper we characterize the interaction between the α subunit of C. perfringens RNA polymerase and the phased A-tracts. Hydroxyl radical footprinting and fluorescence polarization assaying revealed that the α subunit binds to the minor grooves of the phased A-tracts through its C-terminal domain with increased affinity at low temperature. The result provides a molecular mechanism underlying the activation of the plc promoter by the phased A-tracts.

KW - Clostridium perfringens

KW - Curved DNA

KW - Gene expression

KW - Phospholipase C

KW - RNA polymerase

KW - α Subunit

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DO - 10.1016/S0014-5793(01)03148-9

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SN - 0014-5793

VL - 509

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JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Phased A-tracts bind to the α subunit of RNA polymerase with increased affinity at low temperature

Abstract

Keywords

ASJC Scopus subject areas

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