Phenylethylamine induces an increase in cytosolic ca2+ in Yeast

Reinhard Pinontoan, Svetlana Krystofova, Tomonori Kawano, Izumi C. Mori, Frederick I. Tsuji, Hidetoshi Iida, Shoshi Muto

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


β-Phenylethylamine (PEA) induced an increase in cytosolic free calcium ion concentration ([Ca2+]c) in Saccharomyces cereviseae cells monitored with transgenic aequorin, a Ca2+-dependent photoprotein. The PEA-induced [Ca2+]c increase was dependent on the concentrations of PEA applied, and the Ca2+ mostly originated from an extracellular source. Preceding the Ca2+ influx, H2O2 was generated in the cells by the addition of PEA. Externally added H2O2 also induced a [Ca2+]c increase. These results suggest that PEA induces the [Ca2+]c increase via H2O2 generation. The PEA-induced [Ca2+]c increase occurred in the mid1 mutant with a slightly smaller peak than in the wild-type strain, indicating that Mid1, a stretch-activated nonselective cation channel, may not be mainly involved in the PEA-induced Ca2+ influx. When PEA was applied, the MATa mid1 mutant was rescued from α-factor-induced death in a Ca2+-limited medium, suggesting that the PEA-induced [Ca2+]c increase can reinforce calcium signaling in the mating pheromone response pathway.

Original languageEnglish
Pages (from-to)1069-1074
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Issue number5
Publication statusPublished - 2002
Externally publishedYes


  • Calcium signaling
  • Cytosolic Ca
  • Hydrogen peroxide
  • Saccharomyces cerevisiae
  • β-phenylethylamine

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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