Phosphorylation of retinoblastoma protein in rat brain after transient middle cerebral artery occlusion

T. Hayashi, K. Sakai, C. Sasaki, W. R. Zhang, K. Abe

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


Although mature neurones do not replicate genomic DNA, some cell cycle-related kinases are aberrantly activated in neurones after ischaemia. As hyper-phosphorylation of retinoblastoma (Rb) protein is the common pathway in mitotic signal cascade, this study investigated the phosphorylation state of the Rb protein as well as its mRNA level in rat brain after transient middle cerebral artery (MCA) occlusion. Immunohistochemical analysis revealed that neurones in the sham-operated brain expressed Rb protein without the hyperphosphorylated form. Immunoreactivity for the hyperphosphorylated form of Rb protein progressively increased from 1 h to 3 days after ischaemia in neurones in the MCA territory. Western blot analysis demonstrated a similar change. However, reverse transcription-polymerase chain reaction study revealed that Rb showed no definite change at the mRNA level. These results suggest that Rb protein is progressively hyperphosphorylated in the brain after ischaemia, which may activate apoptotic mechanisms in neuronal cells of the brain after ischaemia.

Original languageEnglish
Pages (from-to)390-397
Number of pages8
JournalNeuropathology and Applied Neurobiology
Issue number4
Publication statusPublished - 2000


  • Brain
  • Ischaemia
  • Phosphorylation
  • Rat
  • Retinoblastoma protein

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Neurology
  • Clinical Neurology
  • Physiology (medical)


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