Photo-acceleration of protein release from endosome in the protein transduction system

Masayuki Matsushita; Hirofumi Noguchi; Yun Fei Lu; Kazuhito Tomizawa; Hiroyuki Michiue; Sheng Tian Li; Kenzo Hirose; Susan Bonner-Weir; Hideki Matsui

doi:10.1016/j.febslet.2004.07.033

Photo-acceleration of protein release from endosome in the protein transduction system

Masayuki Matsushita, Hirofumi Noguchi, Yun Fei Lu, Kazuhito Tomizawa, Hiroyuki Michiue, Sheng Tian Li, Kenzo Hirose, Susan Bonner-Weir, Hideki Matsui

Neutron Therapy Research Center

Research output: Contribution to journal › Article › peer-review

69 Citations (Scopus)

Abstract

The protein transduction system has been employed for delivery of bioactive proteins into cells via an endocytotic mechanism. However, trapping of endocytosed proteins in the endosome may significantly attenuate biological actions in cells. The present investigation demonstrated that endosomal release of transduced protein could be artificially accelerated by exposure to fluorescent light. Exposure to light at 480 nm stimulated endosomal release of transduced FITC-11 arginine-protein transduction domain (11R-PTD), TAT-PTD and Antennapedia-PTD. Moreover, FITC-11R-p53 protein was released from endosomes following stimulation with light. These data suggest that photo-acceleration is a more efficient strategy in terms of the protein transduction system.

Original language	English
Pages (from-to)	221-226
Number of pages	6
Journal	FEBS Letters
Volume	572
Issue number	1-3
DOIs	https://doi.org/10.1016/j.febslet.2004.07.033
Publication status	Published - Aug 13 2004

Keywords

Endosomal release
FITC
Photo
Protein transduction
Protein transduction domain

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2004.07.033

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title = "Photo-acceleration of protein release from endosome in the protein transduction system",

abstract = "The protein transduction system has been employed for delivery of bioactive proteins into cells via an endocytotic mechanism. However, trapping of endocytosed proteins in the endosome may significantly attenuate biological actions in cells. The present investigation demonstrated that endosomal release of transduced protein could be artificially accelerated by exposure to fluorescent light. Exposure to light at 480 nm stimulated endosomal release of transduced FITC-11 arginine-protein transduction domain (11R-PTD), TAT-PTD and Antennapedia-PTD. Moreover, FITC-11R-p53 protein was released from endosomes following stimulation with light. These data suggest that photo-acceleration is a more efficient strategy in terms of the protein transduction system.",

keywords = "Endosomal release, FITC, Photo, Protein transduction, Protein transduction domain",

author = "Masayuki Matsushita and Hirofumi Noguchi and Lu, {Yun Fei} and Kazuhito Tomizawa and Hiroyuki Michiue and Li, {Sheng Tian} and Kenzo Hirose and Susan Bonner-Weir and Hideki Matsui",

note = "Funding Information: We thank Dr. T. Jacks (MIT) and Dr. H. Matsushita (Okayama U) for providing the glioma cell line. This research was supported by a Grant-in-Aid for Scientific Research on Priority Areas (C) “medical genome science” from the Ministry of Education, Culture, Sports, Science and Technology of Japan and by NIH grants DK 61251, Research Foundation, the Diabetes Research and Wellness Foundation and an important group of private donors.",

year = "2004",

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doi = "10.1016/j.febslet.2004.07.033",

language = "English",

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AU - Matsushita, Masayuki

AU - Noguchi, Hirofumi

AU - Lu, Yun Fei

AU - Tomizawa, Kazuhito

AU - Michiue, Hiroyuki

AU - Li, Sheng Tian

AU - Hirose, Kenzo

AU - Bonner-Weir, Susan

AU - Matsui, Hideki

N1 - Funding Information: We thank Dr. T. Jacks (MIT) and Dr. H. Matsushita (Okayama U) for providing the glioma cell line. This research was supported by a Grant-in-Aid for Scientific Research on Priority Areas (C) “medical genome science” from the Ministry of Education, Culture, Sports, Science and Technology of Japan and by NIH grants DK 61251, Research Foundation, the Diabetes Research and Wellness Foundation and an important group of private donors.

PY - 2004/8/13

Y1 - 2004/8/13

N2 - The protein transduction system has been employed for delivery of bioactive proteins into cells via an endocytotic mechanism. However, trapping of endocytosed proteins in the endosome may significantly attenuate biological actions in cells. The present investigation demonstrated that endosomal release of transduced protein could be artificially accelerated by exposure to fluorescent light. Exposure to light at 480 nm stimulated endosomal release of transduced FITC-11 arginine-protein transduction domain (11R-PTD), TAT-PTD and Antennapedia-PTD. Moreover, FITC-11R-p53 protein was released from endosomes following stimulation with light. These data suggest that photo-acceleration is a more efficient strategy in terms of the protein transduction system.

AB - The protein transduction system has been employed for delivery of bioactive proteins into cells via an endocytotic mechanism. However, trapping of endocytosed proteins in the endosome may significantly attenuate biological actions in cells. The present investigation demonstrated that endosomal release of transduced protein could be artificially accelerated by exposure to fluorescent light. Exposure to light at 480 nm stimulated endosomal release of transduced FITC-11 arginine-protein transduction domain (11R-PTD), TAT-PTD and Antennapedia-PTD. Moreover, FITC-11R-p53 protein was released from endosomes following stimulation with light. These data suggest that photo-acceleration is a more efficient strategy in terms of the protein transduction system.

KW - Endosomal release

KW - FITC

KW - Photo

KW - Protein transduction

KW - Protein transduction domain

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Photo-acceleration of protein release from endosome in the protein transduction system

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Keywords

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