Photoaffinity labeling of the phylloquinone-binding polypeptides by 2-azidoanthraquinone in photosystem I particles

Masayo Iwaki; Masaaki Takahashi; Keizo Shimada; Yuichiro Takahashi; Shigeru Itoh

doi:10.1016/0014-5793(92)81403-9

Photoaffinity labeling of the phylloquinone-binding polypeptides by 2-azidoanthraquinone in photosystem I particles

Masayo Iwaki, Masaaki Takahashi, Keizo Shimada, Yuichiro Takahashi, Shigeru Itoh

Research Institute for Interdisciplinary Science

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

A photoaffinity label, 2-azido-9,10-anthraquinone, binds at the quinone-binding (Q_φ) site with high affinity and can substitute for the secondary acceptor, phylloquinone, in photosystem I reaction center of spinach. Phylloquinone-depleted photosystem I particles reconstituted with azido-[³H]anthraquinone were illuminated with UV light and analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis. The large core polypeptides (psaA and/or psaB) were selectively labeled. The labeling was competitively inhibited in the presence of anthraquinone. These results indicate that the Q_φ site is located on psaA or psaB polypeptides.

Original language	English
Pages (from-to)	27-30
Number of pages	4
Journal	FEBS Letters
Volume	312
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(92)81403-9
Publication status	Published - Nov 2 1992

Keywords

Electron transfer
Photoaffinity label
Photosystem I
Phylloquinone (vitamin K)
Reaction center

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(92)81403-9

Cite this

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title = "Photoaffinity labeling of the phylloquinone-binding polypeptides by 2-azidoanthraquinone in photosystem I particles",

abstract = "A photoaffinity label, 2-azido-9,10-anthraquinone, binds at the quinone-binding (Qφ) site with high affinity and can substitute for the secondary acceptor, phylloquinone, in photosystem I reaction center of spinach. Phylloquinone-depleted photosystem I particles reconstituted with azido-[3H]anthraquinone were illuminated with UV light and analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis. The large core polypeptides (psaA and/or psaB) were selectively labeled. The labeling was competitively inhibited in the presence of anthraquinone. These results indicate that the Qφ site is located on psaA or psaB polypeptides.",

keywords = "Electron transfer, Photoaffinity label, Photosystem I, Phylloquinone (vitamin K), Reaction center",

author = "Masayo Iwaki and Masaaki Takahashi and Keizo Shimada and Yuichiro Takahashi and Shigeru Itoh",

year = "1992",

month = nov,

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doi = "10.1016/0014-5793(92)81403-9",

language = "English",

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journal = "FEBS Letters",

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T1 - Photoaffinity labeling of the phylloquinone-binding polypeptides by 2-azidoanthraquinone in photosystem I particles

AU - Iwaki, Masayo

AU - Takahashi, Masaaki

AU - Shimada, Keizo

AU - Takahashi, Yuichiro

AU - Itoh, Shigeru

PY - 1992/11/2

Y1 - 1992/11/2

N2 - A photoaffinity label, 2-azido-9,10-anthraquinone, binds at the quinone-binding (Qφ) site with high affinity and can substitute for the secondary acceptor, phylloquinone, in photosystem I reaction center of spinach. Phylloquinone-depleted photosystem I particles reconstituted with azido-[3H]anthraquinone were illuminated with UV light and analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis. The large core polypeptides (psaA and/or psaB) were selectively labeled. The labeling was competitively inhibited in the presence of anthraquinone. These results indicate that the Qφ site is located on psaA or psaB polypeptides.

AB - A photoaffinity label, 2-azido-9,10-anthraquinone, binds at the quinone-binding (Qφ) site with high affinity and can substitute for the secondary acceptor, phylloquinone, in photosystem I reaction center of spinach. Phylloquinone-depleted photosystem I particles reconstituted with azido-[3H]anthraquinone were illuminated with UV light and analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis. The large core polypeptides (psaA and/or psaB) were selectively labeled. The labeling was competitively inhibited in the presence of anthraquinone. These results indicate that the Qφ site is located on psaA or psaB polypeptides.

KW - Electron transfer

KW - Photoaffinity label

KW - Photosystem I

KW - Phylloquinone (vitamin K)

KW - Reaction center

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U2 - 10.1016/0014-5793(92)81403-9

DO - 10.1016/0014-5793(92)81403-9

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SN - 0014-5793

VL - 312

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JO - FEBS Letters

JF - FEBS Letters

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ER -

Photoaffinity labeling of the phylloquinone-binding polypeptides by 2-azidoanthraquinone in photosystem I particles

Abstract

Keywords

ASJC Scopus subject areas

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