TY - JOUR
T1 - Photodynamic therapy with ATX-S10•Na(II) inhibits synovial sarcoma cell growth
AU - Takeda, Ken
AU - Kunisada, Toshiyuki
AU - Miyazawa, Shinichi
AU - Nakae, Yoshinori
AU - Ozaki, Toshifumi
N1 - Funding Information:
One or more of the authors (TK, TO) have received funding from Grants-in-aid for Young Scientists (B) from the Ministry of Education, Culture, Sports, Science and Technology (18791040 and 15790792), by a grant from the Japan Orthopaedics and Traumatology Foundation Inc (0158), by Grants-in-Aid for Clinical Cancer Research and Grants-in-Aid for Cancer Research (14S-4 and -5) from the Ministry of Health, Labor and Welfare, and by a grant from the JSPS Fujita Memorial Fund for Medical Research. Each author certifies that his or her institution has approved the animal protocol for this investigation and that all investigations were conducted in conformity with ethical principles of research.
PY - 2008/7
Y1 - 2008/7
N2 - Photodynamic therapy (PDT) is an effective cancer treatment modality that allows selective destruction of malignant tumor cells. We asked whether PDT could inhibit in vivo and in vitro growth of synovial sarcoma cells. We analyzed PDT using ATX-S10•Na(II) and a diode laser for a synovial sarcoma cell line (SYO-1). Photodynamic therapy with ATX-S10•Na(II) showed an in vitro cytotoxic effect on the cultured SYO-1 cells. The in vitro effect of PDT depended on the treatment concentration of ATX-S10•Na(II) and the laser dose of irradiation. ATX-S10•Na(II) was detected in the tumor tissue specimens that were excised from nude mice bearing SYO-1 within 6 hours after intravenous injection, but it was eliminated from the tumor 12 hours after injection. Photodynamic therapy suppressed the tumor growth of nude mice bearing SYO-1, and high-dose irradiation induced no viable tumor cells in histologic specimens. Photodynamic therapy performed after marginal resection of the tumor of nude mice bearing SYO-1 reduced the rate of local recurrence of the tumor. Our results suggest PDT using ATX-S10•Na(II) and laser irradiation may be a potentially useful treatment for synovial sarcoma, especially to reduce the surgical margin and preserve critical anatomic structures adjacent to the tumor.
AB - Photodynamic therapy (PDT) is an effective cancer treatment modality that allows selective destruction of malignant tumor cells. We asked whether PDT could inhibit in vivo and in vitro growth of synovial sarcoma cells. We analyzed PDT using ATX-S10•Na(II) and a diode laser for a synovial sarcoma cell line (SYO-1). Photodynamic therapy with ATX-S10•Na(II) showed an in vitro cytotoxic effect on the cultured SYO-1 cells. The in vitro effect of PDT depended on the treatment concentration of ATX-S10•Na(II) and the laser dose of irradiation. ATX-S10•Na(II) was detected in the tumor tissue specimens that were excised from nude mice bearing SYO-1 within 6 hours after intravenous injection, but it was eliminated from the tumor 12 hours after injection. Photodynamic therapy suppressed the tumor growth of nude mice bearing SYO-1, and high-dose irradiation induced no viable tumor cells in histologic specimens. Photodynamic therapy performed after marginal resection of the tumor of nude mice bearing SYO-1 reduced the rate of local recurrence of the tumor. Our results suggest PDT using ATX-S10•Na(II) and laser irradiation may be a potentially useful treatment for synovial sarcoma, especially to reduce the surgical margin and preserve critical anatomic structures adjacent to the tumor.
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U2 - 10.1007/s11999-008-0284-6
DO - 10.1007/s11999-008-0284-6
M3 - Article
C2 - 18465181
AN - SCOPUS:45849126190
SN - 0009-921X
VL - 466
SP - 1726
EP - 1733
JO - Clinical orthopaedics and related research
JF - Clinical orthopaedics and related research
IS - 7
ER -
