Photoinduced apoptosis using a peptide carrying a photosensitizer

Kazunori Watanabe; Hayato Fujiwara; Mizuki Kitamatsu; Takashi Ohtsuki

doi:10.1016/j.bmcl.2016.04.091

Photoinduced apoptosis using a peptide carrying a photosensitizer

Kazunori Watanabe, Hayato Fujiwara, Mizuki Kitamatsu, Takashi Ohtsuki

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A novel molecule, TatBim-Alexa, consisting of the HIV1 Tat cell-penetrating peptide, the Bim apoptosis-inducing peptide, and Alexa Fluor 546 was synthesized for photoinducion of apoptosis. The Alexa Fluor 546 was used as a photosensitizer and covalently attached at the C-terminus of TatBim peptide by the thiol-maleimide reaction. Photo-dependent cytosolic internalization of TatBim-Alexa and photo-dependent apoptosis using TatBim-Alexa were demonstrated in several kinds of mammalian cells including human cancer cell lines.

Original language	English
Pages (from-to)	3115-3118
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	26
Issue number	13
DOIs	https://doi.org/10.1016/j.bmcl.2016.04.091
Publication status	Published - Jul 1 2016

Keywords

Apoptosis
Bim
Cell-penetrating peptide
Photosensitizer

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bmcl.2016.04.091

Cite this

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title = "Photoinduced apoptosis using a peptide carrying a photosensitizer",

abstract = "A novel molecule, TatBim-Alexa, consisting of the HIV1 Tat cell-penetrating peptide, the Bim apoptosis-inducing peptide, and Alexa Fluor 546 was synthesized for photoinducion of apoptosis. The Alexa Fluor 546 was used as a photosensitizer and covalently attached at the C-terminus of TatBim peptide by the thiol-maleimide reaction. Photo-dependent cytosolic internalization of TatBim-Alexa and photo-dependent apoptosis using TatBim-Alexa were demonstrated in several kinds of mammalian cells including human cancer cell lines.",

keywords = "Apoptosis, Bim, Cell-penetrating peptide, Photosensitizer",

author = "Kazunori Watanabe and Hayato Fujiwara and Mizuki Kitamatsu and Takashi Ohtsuki",

note = "Funding Information: We thank Dr. Ayano Sato for the material (staurosporine). This work was funded by the JSPS KAKENHI (No. 25282232 ) and Grants-in-Aid for Scientific Research on Innovative Areas {\textquoteleft}Nanomedicine Molecular Science{\textquoteright} to T.O. (26107711). Publisher Copyright: {\textcopyright} 2016 Elsevier Ltd. All rights reserved.",

year = "2016",

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doi = "10.1016/j.bmcl.2016.04.091",

language = "English",

volume = "26",

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journal = "Bioorganic and Medicinal Chemistry Letters",

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T1 - Photoinduced apoptosis using a peptide carrying a photosensitizer

AU - Watanabe, Kazunori

AU - Fujiwara, Hayato

AU - Kitamatsu, Mizuki

AU - Ohtsuki, Takashi

N1 - Funding Information: We thank Dr. Ayano Sato for the material (staurosporine). This work was funded by the JSPS KAKENHI (No. 25282232 ) and Grants-in-Aid for Scientific Research on Innovative Areas ‘Nanomedicine Molecular Science’ to T.O. (26107711). Publisher Copyright: © 2016 Elsevier Ltd. All rights reserved.

PY - 2016/7/1

Y1 - 2016/7/1

N2 - A novel molecule, TatBim-Alexa, consisting of the HIV1 Tat cell-penetrating peptide, the Bim apoptosis-inducing peptide, and Alexa Fluor 546 was synthesized for photoinducion of apoptosis. The Alexa Fluor 546 was used as a photosensitizer and covalently attached at the C-terminus of TatBim peptide by the thiol-maleimide reaction. Photo-dependent cytosolic internalization of TatBim-Alexa and photo-dependent apoptosis using TatBim-Alexa were demonstrated in several kinds of mammalian cells including human cancer cell lines.

AB - A novel molecule, TatBim-Alexa, consisting of the HIV1 Tat cell-penetrating peptide, the Bim apoptosis-inducing peptide, and Alexa Fluor 546 was synthesized for photoinducion of apoptosis. The Alexa Fluor 546 was used as a photosensitizer and covalently attached at the C-terminus of TatBim peptide by the thiol-maleimide reaction. Photo-dependent cytosolic internalization of TatBim-Alexa and photo-dependent apoptosis using TatBim-Alexa were demonstrated in several kinds of mammalian cells including human cancer cell lines.

KW - Apoptosis

KW - Bim

KW - Cell-penetrating peptide

KW - Photosensitizer

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DO - 10.1016/j.bmcl.2016.04.091

M3 - Article

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AN - SCOPUS:84971591990

SN - 0960-894X

VL - 26

SP - 3115

EP - 3118

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 13

ER -

Photoinduced apoptosis using a peptide carrying a photosensitizer

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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