Photosensitizing carrier proteins for photoinducible RNA interference

Yuka Matsushita-Ishiodori; Rina Kuwabara; Hiroyuki Sakakoshi; Tamaki Endoh; Takashi Ohtsuki

doi:10.1021/bc200095a

Photosensitizing carrier proteins for photoinducible RNA interference

Yuka Matsushita-Ishiodori, Rina Kuwabara, Hiroyuki Sakakoshi, Tamaki Endoh, Takashi Ohtsuki

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

RNA interference (RNAi) is being widely explored as a tool in functional genomics and tissue engineering, and in the therapy of intractable diseases, including cancer and neurodegenerative diseases. Recently, we developed a photoinducible RNAi method using photosensitizing carrier proteins, named CLIP-RNAi (CPP-linked RBP-mediated RNA internalization and photoinduced RNAi). Novel carrier proteins were designed for this study to establish a highly efficient delivery system for small interfering RNA (siRNA) or short hairpin RNA (shRNA) and to demonstrate light-dependent gene silencing. In addition, the results suggested that the dissociation of the siRNA (or shRNA) from carrier proteins in the cytoplasm is a critical event in CLIP-RNAi-mediated gene silencing.

Original language	English
Pages (from-to)	2222-2226
Number of pages	5
Journal	Bioconjugate Chemistry
Volume	22
Issue number	11
DOIs	https://doi.org/10.1021/bc200095a
Publication status	Published - Nov 16 2011

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1021/bc200095a

Cite this

@article{e5abd9d41c63495d8dc5d7cce3336b3b,

title = "Photosensitizing carrier proteins for photoinducible RNA interference",

abstract = "RNA interference (RNAi) is being widely explored as a tool in functional genomics and tissue engineering, and in the therapy of intractable diseases, including cancer and neurodegenerative diseases. Recently, we developed a photoinducible RNAi method using photosensitizing carrier proteins, named CLIP-RNAi (CPP-linked RBP-mediated RNA internalization and photoinduced RNAi). Novel carrier proteins were designed for this study to establish a highly efficient delivery system for small interfering RNA (siRNA) or short hairpin RNA (shRNA) and to demonstrate light-dependent gene silencing. In addition, the results suggested that the dissociation of the siRNA (or shRNA) from carrier proteins in the cytoplasm is a critical event in CLIP-RNAi-mediated gene silencing.",

author = "Yuka Matsushita-Ishiodori and Rina Kuwabara and Hiroyuki Sakakoshi and Tamaki Endoh and Takashi Ohtsuki",

year = "2011",

month = nov,

day = "16",

doi = "10.1021/bc200095a",

language = "English",

volume = "22",

pages = "2222--2226",

journal = "Bioconjugate Chemistry",

issn = "1043-1802",

publisher = "American Chemical Society",

number = "11",

}

TY - JOUR

T1 - Photosensitizing carrier proteins for photoinducible RNA interference

AU - Matsushita-Ishiodori, Yuka

AU - Kuwabara, Rina

AU - Sakakoshi, Hiroyuki

AU - Endoh, Tamaki

AU - Ohtsuki, Takashi

PY - 2011/11/16

Y1 - 2011/11/16

N2 - RNA interference (RNAi) is being widely explored as a tool in functional genomics and tissue engineering, and in the therapy of intractable diseases, including cancer and neurodegenerative diseases. Recently, we developed a photoinducible RNAi method using photosensitizing carrier proteins, named CLIP-RNAi (CPP-linked RBP-mediated RNA internalization and photoinduced RNAi). Novel carrier proteins were designed for this study to establish a highly efficient delivery system for small interfering RNA (siRNA) or short hairpin RNA (shRNA) and to demonstrate light-dependent gene silencing. In addition, the results suggested that the dissociation of the siRNA (or shRNA) from carrier proteins in the cytoplasm is a critical event in CLIP-RNAi-mediated gene silencing.

AB - RNA interference (RNAi) is being widely explored as a tool in functional genomics and tissue engineering, and in the therapy of intractable diseases, including cancer and neurodegenerative diseases. Recently, we developed a photoinducible RNAi method using photosensitizing carrier proteins, named CLIP-RNAi (CPP-linked RBP-mediated RNA internalization and photoinduced RNAi). Novel carrier proteins were designed for this study to establish a highly efficient delivery system for small interfering RNA (siRNA) or short hairpin RNA (shRNA) and to demonstrate light-dependent gene silencing. In addition, the results suggested that the dissociation of the siRNA (or shRNA) from carrier proteins in the cytoplasm is a critical event in CLIP-RNAi-mediated gene silencing.

UR - http://www.scopus.com/inward/record.url?scp=81255138076&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=81255138076&partnerID=8YFLogxK

U2 - 10.1021/bc200095a

DO - 10.1021/bc200095a

M3 - Article

C2 - 21985108

AN - SCOPUS:81255138076

SN - 1043-1802

VL - 22

SP - 2222

EP - 2226

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

IS - 11

ER -

Photosensitizing carrier proteins for photoinducible RNA interference

Abstract

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this