Physical mapping of plasmid and cosmid clones in filamentous fungi by fiber-FISH

Dai Tsuchiya, Aya Matsumoto, Sarah F. Covert, Charlotte R. Bronson, Masatoki Taga

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Fluorescence in situ hybridization to extended DNA fibers (fiber-FISH) serves as a powerful tool for direct physical mapping in plants and animals. Here, we show that fiber-FISH is useful for contig mapping as well as for estimating the physical distance between genetic markers in fungi. A five-cosmid contig from a chromosome of Nectria haematococca and four cloned genetic markers from a linkage map of Cochliobolus heterostrophus were chosen as models for the application of this technology. In N. haematococca, overlapping and non-overlapping clones were visually mapped on individual DNA fibers, confirming the results from conventional physical mapping perfectly. Fiber-FISH concomitantly indicated the gap size or the extent of overlap between two clones. In C. heterostrophus, the physical distance between the two pairs of genetic markers could be estimated from the microscopic measurements of the intervals. Chromosomal DNA isolated from a pulsed field gel was suitable for preparing the DNA fibers.

Original languageEnglish
Pages (from-to)22-28
Number of pages7
JournalFungal Genetics and Biology
Issue number1
Publication statusPublished - 2002
Externally publishedYes


  • Chromosome
  • Cochliobolus heterostrophus
  • Contig
  • Fiber-FISH
  • Fluorescence in situ hybridization
  • Nectria haematococca
  • Physical mapping

ASJC Scopus subject areas

  • Microbiology
  • Genetics


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