Pivotal role of STIM2, but not STIM1, in IL-4 production by IL-3–stimulated murine basophils

Soichiro Yoshikawa, Masatsugu Ohhora, Ryota Hashimoto, Toshihisa Nagao, Louis Peters, Mayumi Egawa, Takuya Ohta, Kensuke Miyake, Takahiro Adachi, Yohei Kawano, Yoshinori Yamanishi, Hajime Karasuyama

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Basophils have nonredundant roles in various immune responses that require Ca 2+ influx. Here, we examined the role of two Ca 2+ sensors, stromal interaction molecule 1 and 2 (STIM1 and STIM2), in basophil activation. We found that loss of STIM1, but not STIM2, impaired basophil IL-4 production after stimulation with immunoglobulin E (IgE)–containing immune complexes. In contrast, when basophils were stimulated with IL-3, loss of STIM2, but not STIM1, reduced basophil IL-4 production. This difference in STIM proteins was associated with distinct time courses of Ca 2+ influx and transcription of the Il4 gene that were elicited by each stimulus. Similarly, basophil-specific STIM1 expression was required for IgE-driven chronic allergic inflammation in vivo, whereas STIM2 was required for IL-4 production after combined IL-3 and IL-33 treatment in mice. These data indicate that STIM1 and STIM2 have differential roles in the production of IL-4, which are stimulus dependent. Furthermore, these results illustrate the vital role of STIM2 in basophils, which is often considered to be less important than STIM1.

Original languageEnglish
Article numbereaav2060
JournalScience Signaling
Issue number576
Publication statusPublished - Apr 9 2019
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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