TY - JOUR
T1 - Polymorphisms detected in actin-related sequences of rats (Rattus norvegicus).
AU - Kunieda, T.
AU - Ikadai, H.
AU - Matsui, M.
AU - Nomura, N.
AU - Ishizaki, R.
AU - Imamichi, T.
PY - 1990/4
Y1 - 1990/4
N2 - Southern blot hybridization of EcoRI digests of DNAs from 13 rat strains using human cardiac actin gene as a probe revealed polymorphisms in actin-related sequences of rats. EcoRI fragments of 11 kb, 7 kb, 6 kb, 5 kb, 4.5 kb and 4 kb detected in several strains were absent in the remaining strains. The presence of these fragments was suggested to be due to presence of extra sequences homologous to the actin genes, such as processed pseudogenes, in the particular strains. The 13 strains were assigned to each of 7 specific patterns of the polymorphic EcoRI fragments. It was concluded that the polymorphisms of actin-related sequences should be useful for genetic monitoring of laboratory rats.
AB - Southern blot hybridization of EcoRI digests of DNAs from 13 rat strains using human cardiac actin gene as a probe revealed polymorphisms in actin-related sequences of rats. EcoRI fragments of 11 kb, 7 kb, 6 kb, 5 kb, 4.5 kb and 4 kb detected in several strains were absent in the remaining strains. The presence of these fragments was suggested to be due to presence of extra sequences homologous to the actin genes, such as processed pseudogenes, in the particular strains. The 13 strains were assigned to each of 7 specific patterns of the polymorphic EcoRI fragments. It was concluded that the polymorphisms of actin-related sequences should be useful for genetic monitoring of laboratory rats.
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U2 - 10.1538/expanim1978.39.2_307
DO - 10.1538/expanim1978.39.2_307
M3 - Article
C2 - 2361532
AN - SCOPUS:0025409750
SN - 0007-5124
VL - 39
SP - 307
EP - 310
JO - Jikken dobutsu. Experimental animals
JF - Jikken dobutsu. Experimental animals
IS - 2
ER -