TY - JOUR
T1 - Polymorphisms in the helicobacter pylori NY43 strain and its prophage-cured derivatives
AU - Takeuchi, Hiroaki
AU - Kira, Mizuki
AU - Konishi, Sayuri
AU - Uchiyama, Jumpei
AU - Matsuzaki, Shigenobu
AU - Matsumura, Yoshihisa
N1 - Funding Information:
This work was supported by JSPS KEKENHI grant numbers 15K08618 and 15K08617.
Publisher Copyright:
© 2018 The Authors.
PY - 2018/6
Y1 - 2018/6
N2 - This study aimed to determine the characteristics of the Helicobacter pylori host NY43 strain and its prophage-cured derivative. H. pylori colonizing the human stomach cause many diseases. They show high genetic diversity, allowing the development of mutant strains that can form bacterial communities adapted to specific environmental conditions. Bacteriophage activities are associated with bacterial evolution, including pathogenicity development. Herein, we reported the complete genome sequence and genomic organization of two H. pylori prophages, KHP30 and KHP40; the effects of KHP30 on the behaviours of NY43 are not yet known. We showed that approximately 57% prophage-cured derivatives spontaneously appeared in the exponential phase during liquid culture, and the biological characteristics of these derivatives differed from those of the host NY43. KHP30 reinfected the cured derivatives, and the curing ratio was influenced by culture conditions. KHP30 was shown to promote the development of a flexible H. pylori community with variable characteristics.
AB - This study aimed to determine the characteristics of the Helicobacter pylori host NY43 strain and its prophage-cured derivative. H. pylori colonizing the human stomach cause many diseases. They show high genetic diversity, allowing the development of mutant strains that can form bacterial communities adapted to specific environmental conditions. Bacteriophage activities are associated with bacterial evolution, including pathogenicity development. Herein, we reported the complete genome sequence and genomic organization of two H. pylori prophages, KHP30 and KHP40; the effects of KHP30 on the behaviours of NY43 are not yet known. We showed that approximately 57% prophage-cured derivatives spontaneously appeared in the exponential phase during liquid culture, and the biological characteristics of these derivatives differed from those of the host NY43. KHP30 reinfected the cured derivatives, and the curing ratio was influenced by culture conditions. KHP30 was shown to promote the development of a flexible H. pylori community with variable characteristics.
KW - Biological polymorphism
KW - Helicobacter pylori
KW - Host strain (NY43)
KW - Novel prophage-cured derivatives
KW - Prophage (KHP30)
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UR - http://www.scopus.com/inward/citedby.url?scp=85048141340&partnerID=8YFLogxK
U2 - 10.1099/mic.0.000665
DO - 10.1099/mic.0.000665
M3 - Article
C2 - 29738305
AN - SCOPUS:85048141340
SN - 1350-0872
VL - 164
SP - 877
EP - 882
JO - Microbiology (United Kingdom)
JF - Microbiology (United Kingdom)
IS - 6
M1 - 000665
ER -
