Possible coupling of prostaglandin E receptor EP1 to TRP5 expressed in Xenopus laevis oocytes

Hiroyuki Tabata; Satoshi Tanaka; Yukihiko Sugimoto; Hideaki Kanki; Shuji Kaneko; Atsushi Ichikawa

doi:10.1016/S0006-291X(02)02455-5

Possible coupling of prostaglandin E receptor EP₁ to TRP5 expressed in Xenopus laevis oocytes

Hiroyuki Tabata, Satoshi Tanaka, Yukihiko Sugimoto, Hideaki Kanki, Shuji Kaneko, Atsushi Ichikawa

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

We previously reported that the prostaglandin E₂ (PGE₂) receptor subtype EP₁ is coupled to intracellular Ca²⁺ mobilization in CHO cells, which is dependent on extracellular Ca²⁺ in a pertussis toxin-insensitive manner [H. Katoh, et al., Biochim. Biophys. Acta 1244 (1995) 41-48]. However, it remains unknown about the signal transduction involved in this response. To investigate the mechanism regulating Ca²⁺ mobilization mediated by EP₁ receptors in detail, we performed a series of experiments using the Xenopus laevis oocyte expression system and found that endogenous G_q and/or G₁₁, and not G_i1 is involved in the Ca²⁺ mobilization induced by PGE₂. We further investigated the receptor-activated Ca²⁺ channel (RACC)-related response by introducing mRNA for mouse transient receptor potential 5 (TRP5), a possible candidate for the RACC, and found effective coupling between them. These results suggest that the EP₁ receptors induce Ca²⁺ mobilization via G_q and/or G₁₁ and Ca²⁺ influx via TRP.

Original language	English
Pages (from-to)	398-402
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	298
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(02)02455-5
Publication status	Published - 2002

Keywords

Ca mobilization
EP
Receptor-activated Ca channel
Transient receptor potential

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(02)02455-5

Cite this

@article{834d013195264286ad4f78163b8a9aca,

title = "Possible coupling of prostaglandin E receptor EP1 to TRP5 expressed in Xenopus laevis oocytes",

abstract = "We previously reported that the prostaglandin E2 (PGE2) receptor subtype EP1 is coupled to intracellular Ca2+ mobilization in CHO cells, which is dependent on extracellular Ca2+ in a pertussis toxin-insensitive manner [H. Katoh, et al., Biochim. Biophys. Acta 1244 (1995) 41-48]. However, it remains unknown about the signal transduction involved in this response. To investigate the mechanism regulating Ca2+ mobilization mediated by EP1 receptors in detail, we performed a series of experiments using the Xenopus laevis oocyte expression system and found that endogenous Gq and/or G11, and not Gi1 is involved in the Ca2+ mobilization induced by PGE2. We further investigated the receptor-activated Ca2+ channel (RACC)-related response by introducing mRNA for mouse transient receptor potential 5 (TRP5), a possible candidate for the RACC, and found effective coupling between them. These results suggest that the EP1 receptors induce Ca2+ mobilization via Gq and/or G11 and Ca2+ influx via TRP.",

keywords = "Ca mobilization, EP, Receptor-activated Ca channel, Transient receptor potential",

author = "Hiroyuki Tabata and Satoshi Tanaka and Yukihiko Sugimoto and Hideaki Kanki and Shuji Kaneko and Atsushi Ichikawa",

note = "Funding Information: We thank Ms. A. Popiel for her help in preparation of the manuscript. This study was supported by grants-in-aid for Scientific Research from the Ministry of Education, Science, Sports and Culture, in Japan.",

year = "2002",

doi = "10.1016/S0006-291X(02)02455-5",

language = "English",

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T1 - Possible coupling of prostaglandin E receptor EP1 to TRP5 expressed in Xenopus laevis oocytes

AU - Tabata, Hiroyuki

AU - Tanaka, Satoshi

AU - Sugimoto, Yukihiko

AU - Kanki, Hideaki

AU - Kaneko, Shuji

AU - Ichikawa, Atsushi

N1 - Funding Information: We thank Ms. A. Popiel for her help in preparation of the manuscript. This study was supported by grants-in-aid for Scientific Research from the Ministry of Education, Science, Sports and Culture, in Japan.

PY - 2002

Y1 - 2002

N2 - We previously reported that the prostaglandin E2 (PGE2) receptor subtype EP1 is coupled to intracellular Ca2+ mobilization in CHO cells, which is dependent on extracellular Ca2+ in a pertussis toxin-insensitive manner [H. Katoh, et al., Biochim. Biophys. Acta 1244 (1995) 41-48]. However, it remains unknown about the signal transduction involved in this response. To investigate the mechanism regulating Ca2+ mobilization mediated by EP1 receptors in detail, we performed a series of experiments using the Xenopus laevis oocyte expression system and found that endogenous Gq and/or G11, and not Gi1 is involved in the Ca2+ mobilization induced by PGE2. We further investigated the receptor-activated Ca2+ channel (RACC)-related response by introducing mRNA for mouse transient receptor potential 5 (TRP5), a possible candidate for the RACC, and found effective coupling between them. These results suggest that the EP1 receptors induce Ca2+ mobilization via Gq and/or G11 and Ca2+ influx via TRP.

AB - We previously reported that the prostaglandin E2 (PGE2) receptor subtype EP1 is coupled to intracellular Ca2+ mobilization in CHO cells, which is dependent on extracellular Ca2+ in a pertussis toxin-insensitive manner [H. Katoh, et al., Biochim. Biophys. Acta 1244 (1995) 41-48]. However, it remains unknown about the signal transduction involved in this response. To investigate the mechanism regulating Ca2+ mobilization mediated by EP1 receptors in detail, we performed a series of experiments using the Xenopus laevis oocyte expression system and found that endogenous Gq and/or G11, and not Gi1 is involved in the Ca2+ mobilization induced by PGE2. We further investigated the receptor-activated Ca2+ channel (RACC)-related response by introducing mRNA for mouse transient receptor potential 5 (TRP5), a possible candidate for the RACC, and found effective coupling between them. These results suggest that the EP1 receptors induce Ca2+ mobilization via Gq and/or G11 and Ca2+ influx via TRP.

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KW - Transient receptor potential

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