TY - JOUR
T1 - Possible involvement of protein kinase C and low molecular weight GTP-binding proteins in thrombin-induced histamine secretion in human platelets
AU - Uehara, Takashi
AU - Kitayama, Yoshinon
AU - Kitamura, Yoshihisa
AU - Nomura, Yasuyuki
PY - 1995/1/17
Y1 - 1995/1/17
N2 - Thrombin and 12-O-tetradecanoylphorbol 13-acetate (TPA) caused histamine secretion from human platelets. To clarify the intracellular signalling mechanism of thrombin-induced histamine secretion, the effects of pertussis toxin (PTX) and botulinus toxin (BTX) on thrombin- and TPA-induced histamine secretion were examined in human platelets. The secretion by thrombin was sensitive to BTX, but not PTX. The secretion by TPA was also inhibited by BTX. These results suggest that protein kinase C and low molecular weight G-proteins sensitive to BTX are involved in histamine secretion.
AB - Thrombin and 12-O-tetradecanoylphorbol 13-acetate (TPA) caused histamine secretion from human platelets. To clarify the intracellular signalling mechanism of thrombin-induced histamine secretion, the effects of pertussis toxin (PTX) and botulinus toxin (BTX) on thrombin- and TPA-induced histamine secretion were examined in human platelets. The secretion by thrombin was sensitive to BTX, but not PTX. The secretion by TPA was also inhibited by BTX. These results suggest that protein kinase C and low molecular weight G-proteins sensitive to BTX are involved in histamine secretion.
UR - http://www.scopus.com/inward/record.url?scp=0028966066&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028966066&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1995.1070
DO - 10.1006/bbrc.1995.1070
M3 - Article
C2 - 7529999
AN - SCOPUS:0028966066
SN - 0006-291X
VL - 206
SP - 492
EP - 496
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -