TY - JOUR
T1 - Postnatal Runx2 deletion leads to low bone mass and adipocyte accumulation in mice bone tissues
AU - Tosa, Ikue
AU - Yamada, Daisuke
AU - Yasumatsu, Misa
AU - Hinoi, Eiichi
AU - Ono, Mitsuaki
AU - Oohashi, Toshitaka
AU - Kuboki, T.
AU - Takarada, Takeshi
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Scientific Research to T.T ( 26460387 ). from the Ministry of Education, Culture, Sports, Science and Technology, Japan , and in part by research grants to T.T. from the Takeda Science Foundation .
Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/9/3
Y1 - 2019/9/3
N2 - Global gene deletion studies have established that Runt-related transcription factor-2 (Runx2) is essential during skeletogenesis for osteoblastic differentiation in both intramembranous and endochondral ossification processes. However, the postnatal significance of Runx2 in vivo is poorly understood because a global Runx2 deletion causes perinatal lethality. In this study, we generated tamoxifen-induced Runx2 global deficient mice by crossing Runx2flox mice with ROSA26-CreERT2 mice (Rosa26-CreERT2; Runx2flox/flox). Four-week-old mice were intraperitoneally treated with tamoxifen for five consecutive days, sacrificed, and analyzed six weeks after tamoxifen administration. Deletion of Runx2 led to low bone mass, which is associated with decreased bone formation and bone resorption as well as excessive bone marrow adiposity. Collectively, postnatal Runx2 absolutely plays an important role in maintaining the homeostasis of bone tissues not only in bone mass, but also in the bone marrow environment.
AB - Global gene deletion studies have established that Runt-related transcription factor-2 (Runx2) is essential during skeletogenesis for osteoblastic differentiation in both intramembranous and endochondral ossification processes. However, the postnatal significance of Runx2 in vivo is poorly understood because a global Runx2 deletion causes perinatal lethality. In this study, we generated tamoxifen-induced Runx2 global deficient mice by crossing Runx2flox mice with ROSA26-CreERT2 mice (Rosa26-CreERT2; Runx2flox/flox). Four-week-old mice were intraperitoneally treated with tamoxifen for five consecutive days, sacrificed, and analyzed six weeks after tamoxifen administration. Deletion of Runx2 led to low bone mass, which is associated with decreased bone formation and bone resorption as well as excessive bone marrow adiposity. Collectively, postnatal Runx2 absolutely plays an important role in maintaining the homeostasis of bone tissues not only in bone mass, but also in the bone marrow environment.
KW - Aging
KW - Bone marrow adiposity
KW - Conditional knockout
KW - Osteoporosis
KW - Runx2
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U2 - 10.1016/j.bbrc.2019.07.014
DO - 10.1016/j.bbrc.2019.07.014
M3 - Article
C2 - 31300199
AN - SCOPUS:85068469723
SN - 0006-291X
VL - 516
SP - 1229
EP - 1233
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -