TY - JOUR
T1 - Potential high virulence for infective endocarditis in Streptococcus mutans strains with collagen-binding proteins but lacking PA expression
AU - Nomura, R.
AU - Naka, S.
AU - Nemoto, H.
AU - Otsugu, M.
AU - Nakamura, S.
AU - Ooshima, T.
AU - Nakano, K.
N1 - Funding Information:
This study was supported by a Grant-in-Aid for Scientific Research (B) 23390472 and (B) 24390461 from the Japan Society for Promotion of Science . We thank Prof. Howard K. Kuramitsu (State University of New York at Buffalo) for assistance in editing this manuscript.
PY - 2013
Y1 - 2013
N2 - Objective Streptococcus mutans, an aetiologic agent of dental caries, is a pathogen for infective endocarditis (IE). We investigated strains that express collagen-binding proteins (CBPs) with further classification based on expression of the 190-kDa protein antigen (PA). Method Zeta-potential values of strains TW871 (CBP+/PA+) and MT8148 (CBP-/PA+), and their respective PA-defective mutant strains TW871PD (CBP+/PA-) and MT8148PD (CBP-/PA-), were analysed, as were their adhesion to and invasion of human umbilical vein endothelial cells (HUVECs). The distribution of strains from the oral cavities of 200 healthy individuals was analysed for CBP and/or PA expression and the strains were characterised for their adhesion and invasion properties. Results TW871PD and MT8148PD showed significantly lower zeta-potential values than TW871 and MT8148, respectively. Collagen-binding rates were significantly higher for TW871PD than for TW871 but nearly negligible for MT8148 and MT8148PD. The adhesion and invasion rates of HUVECs were significantly higher for TW871PD than for TW871 and significantly higher for TW871 than for MT8148 and MT8148PD. The prevalence of CBP+ strains was ∼10% and ∼3% in the case of CBP+/PA- strains. Analyses of 200 clinical strains showed the CBP+/PA- group to have higher adhesion and invasion rates than other groups. Conclusions CBP+/PA- S. mutans strains, despite their low distribution frequency, may be highly virulent for infective endocarditis.
AB - Objective Streptococcus mutans, an aetiologic agent of dental caries, is a pathogen for infective endocarditis (IE). We investigated strains that express collagen-binding proteins (CBPs) with further classification based on expression of the 190-kDa protein antigen (PA). Method Zeta-potential values of strains TW871 (CBP+/PA+) and MT8148 (CBP-/PA+), and their respective PA-defective mutant strains TW871PD (CBP+/PA-) and MT8148PD (CBP-/PA-), were analysed, as were their adhesion to and invasion of human umbilical vein endothelial cells (HUVECs). The distribution of strains from the oral cavities of 200 healthy individuals was analysed for CBP and/or PA expression and the strains were characterised for their adhesion and invasion properties. Results TW871PD and MT8148PD showed significantly lower zeta-potential values than TW871 and MT8148, respectively. Collagen-binding rates were significantly higher for TW871PD than for TW871 but nearly negligible for MT8148 and MT8148PD. The adhesion and invasion rates of HUVECs were significantly higher for TW871PD than for TW871 and significantly higher for TW871 than for MT8148 and MT8148PD. The prevalence of CBP+ strains was ∼10% and ∼3% in the case of CBP+/PA- strains. Analyses of 200 clinical strains showed the CBP+/PA- group to have higher adhesion and invasion rates than other groups. Conclusions CBP+/PA- S. mutans strains, despite their low distribution frequency, may be highly virulent for infective endocarditis.
KW - Classification
KW - Collagen-binding protein
KW - Infective endocarditis
KW - Protein antigen (PA)
KW - Streptococcus mutans
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U2 - 10.1016/j.archoralbio.2013.06.008
DO - 10.1016/j.archoralbio.2013.06.008
M3 - Article
C2 - 24112728
AN - SCOPUS:84883876678
SN - 0003-9969
VL - 58
SP - 1627
EP - 1634
JO - Archives of Oral Biology
JF - Archives of Oral Biology
IS - 11
ER -
