Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction

Amy C. Luce; Anupma Sharma; Oliver S.B. Mollere; Thomas K. Wolfgruber; Kiyotaka Nagaki; Jiming Jiang; Gernot G. Presting; R. Kelly Dawe

doi:10.1534/genetics.106.060467

Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction

Amy C. Luce, Anupma Sharma, Oliver S.B. Mollere, Thomas K. Wolfgruber, Kiyotaka Nagaki, Jiming Jiang, Gernot G. Presting, R. Kelly Dawe

Institute of Plant Science and Resources

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat junction mapping was combined with anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8.

Original language	English
Pages (from-to)	1057-1061
Number of pages	5
Journal	Genetics
Volume	174
Issue number	2
DOIs	https://doi.org/10.1534/genetics.106.060467
Publication status	Published - 2006

ASJC Scopus subject areas

Genetics

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abstract = "Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat junction mapping was combined with anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8.",

author = "Luce, {Amy C.} and Anupma Sharma and Mollere, {Oliver S.B.} and Wolfgruber, {Thomas K.} and Kiyotaka Nagaki and Jiming Jiang and Presting, {Gernot G.} and Dawe, {R. Kelly}",

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T1 - Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction

AU - Luce, Amy C.

AU - Sharma, Anupma

AU - Mollere, Oliver S.B.

AU - Wolfgruber, Thomas K.

AU - Nagaki, Kiyotaka

AU - Jiang, Jiming

AU - Presting, Gernot G.

AU - Dawe, R. Kelly

PY - 2006

Y1 - 2006

N2 - Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat junction mapping was combined with anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8.

AB - Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that junctions between repeats provide reliable single-copy markers for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat junction mapping was combined with anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8.

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Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction

Abstract

ASJC Scopus subject areas

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